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P9577

Sigma-Aldrich

PCR 50 - 2,000 bp Marker

for electrophoresis of PCR fragments

Synonym(s):

50-2000 bp ladder, PCR ladder for agarose gel electrophoresis, PCR marker for agarose gel electrophoresis, RTU PCR DNA ladder, RTU PCR DNA marker, ready to use DNA ladder, ready to use DNA marker

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About This Item

UNSPSC Code:
41105337
NACRES:
NA.25

form

liquid

usage

sufficient for 50 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.

Components

Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:
  • 1 vial (250 μl volume) PCR Marker
  • 1 vial 6× loading buffer (250 μl volume) for diluting samples

Other Notes

Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class Code

10 - Combustible liquids

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Undergraduate virology exercises demonstrate conventional and real-time PCR using commercially available HIV primers and noninfectious target.
Sulzinski MA, et al.
Biochemistry and Molecular Biology Education, 37, 232-235 (2009)

Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protocols

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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