Sigma′s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.[1]
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
Ready-to-load
Easy-to-use
Popular band sizes
Components
Sigma′s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
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Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
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