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Millipore

Immobilon® Classico Western HRP substrate

Western blotting, dot/slot blotting

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

product name

Immobilon Classico Western HRP substrate, Western blotting, dot/slot blotting

conjugate

peroxidase conjugate

Quality Level

200

manufacturer/tradename

Luminata

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

Related Categories

General description

Premixed, ready to use chemiluminescent HRP detection reagent

Application

For use on immunoblots.
Western blotting, dot/slot blotting

Packaging

1 bottle for 1000 cm2

Quality

The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.

Storage and Stability

Stable for 1 year at 4°C from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.

Legal Information

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts.
Recolin, Benedicte and Maiorano, Domenico
Biochemical and biophysical research communications (2012)

Articles

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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