sufficient for ≥1,000 tests (150 ml substrate solution)
storage temp.
2-8°C
Application
AEC Staining Kit has been used as a substrate
for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods[1]
for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay[2]
for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads[3]
Packaging
Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.
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Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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