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Merck

G6639

Sigma-Aldrich

Guanidine thiocyanate

≥97% (titration)

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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100 G
SEK 2,490.00

SEK 2,490.00


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100 G
SEK 2,490.00

About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.55

SEK 2,490.00


Availability
Please contact Customer Service for Availability

To order products, please contact your local dealer.

biological source

synthetic

Quality Level

Assay

≥97% (titration)

form

powder or crystals

technique(s)

RNA extraction: suitable

color

white to off-white

mp

120-122 °C (lit.)

solubility

water: 4 M, clear, colorless

anion traces

chloride (Cl-): ≤2.5%

SMILES string

SC#N.NC(N)=N

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This Item
G927750981368975
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

powder or crystals

form

powder or crystals

form

powder

form

solid

assay

≥97% (titration)

assay

≥99%

assay

≥99%

assay

≥99% (titration)

biological source

synthetic

biological source

synthetic (organic)

biological source

synthetic

biological source

-

solubility

water: 4 M, clear, colorless

solubility

water: 100 mg/mL, clear, colorless

solubility

H2O: 4 M at 20 °C, clear, colorless

solubility

water: 25%, ethanol: 5%

Application

Guanidine thiocyanate has been used:
  • as a denaturing agent to study its effects on surface activity of globular protein[1]
  • for the extraction of total RNA from primary hepatocytes[2]
  • as a component of the incubating buffer for extraction of DNA from human bone[3]
  • as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA[4]
  • as a component of lysis solution for extraction of RNA from rat pancreatic tissues[5]
Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases.[6] It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes.[7] GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.[6]

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Emilia Bramanti et al.
Analytical biochemistry, 351(1), 100-113 (2006-01-28)
In this article, a multidimensional dynamic surface tension detector (DSTD), in a parallel configuration with a UV-visible diode array absorbance detector, is presented in a novel flow injection analysis (FIA) application to study the effects of chemical denaturants urea, guanidinium
DNA fromMycobacterium tuberculosisIdentified in Mediaeval Human Skeletal Remains Using Polymerase Chain Reaction
Taylor M G, et al.
Journal of Archaeological Science, 23, 789-798 (1996)
Extraction and Purification of Nucleic Acid using CBNP and PCIA Technique
Soni N
PharmaTutor, 4, 20-22 (2016)
Angela Molinaro et al.
Cell metabolism, 29(6), 1400-1409 (2019-04-16)
Phosphatidylinositol-3-kinase (PI3K) activity is aberrant in tumors, and PI3K inhibitors are investigated as cancer therapeutics. PI3K signaling mediates insulin action in metabolism, but the role of PI3K isoforms in insulin signaling remains unresolved. Defining the role of PI3K isoforms in
Julian R Peat et al.
F1000Research, 6, 526-526 (2017-06-06)
Methylation of CG dinucleotides constitutes a critical system of epigenetic memory in bony vertebrates, where it modulates gene expression and suppresses transposon activity. The genomes of studied vertebrates are pervasively hypermethylated, with the exception of regulatory elements such as transcription

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