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About This Item
NACRES:
NA.26
UNSPSC Code:
12352202
Pricing and availability is not currently available.
General description
The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.
Application
Suitable for the measurement of alkaline phosphatase activity in a variety of samples
Alkaline Phosphatase Diethanolamine Activity Kit has been used:
- in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
- for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
- for determining ALP activity in the bone metastasis model of lung cancer
- for measuring ALP levels in colon tissue homogenates
Biochem/physiol Actions
Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.
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This Item | |||
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| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| usage sufficient for 100 tests | usage kit sufficient for 300 reactions (200 μl reactions) | usage sufficient for 100 tests | usage sufficient for 100 fluorometric tests |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
signalword
Danger
target_organs
Kidney,Liver,Blood
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 2 Oral
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| AP0100-1KT | 04061833418796 |





