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Merck

T6763

Sigma-Aldrich

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

Sinónimos:

Acetyltrypsin

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About This Item

Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

bovine pancreas

Nivel de calidad

tpo

Type V-S

Formulario

powder

actividad específica

≥8,500 BAEE units/mg protein (biuret)

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

Descripción general

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Aplicación

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

Acciones bioquímicas o fisiológicas

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

Definición de unidad

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Nota de preparación

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

Otras notas

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

Pictogramas

Health hazardExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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The study of membrane proteins remains a challenging task, and approaches to unravel their dynamics are scarce. Here, we applied hydrogen/deuterium exchange (HDX) coupled to mass spectrometry to probe the motions of a bacterial multidrug ATP-binding cassette (ABC) transporter, BmrA

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