TLC Analysis Of Plant Extracts on HPTLC Silica gel 60 NH2 F254s

sample preparation

2 g plant material extracted for 1 h at 60°C under shaking with 50 ml solvent. Filtration of crude extract with an 0.45 μm syringe filter. Application by CAMAG ATS4 6 mm bandwise.

plate

1.15647 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151647.0001)

eluent

[A] ethylacetat; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C:D) containing 0.1% TFA

migration distance

6 cm

chamber

normal chamber without chamber saturation

sample

0.150 μL

detector

UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression) (CMS mass spectrometer (+)-ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA.)