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HPLC Analysis of Herbal Supplement Containing Milk Thistle on Ascentis® Express 2.7 μm C18

HPLC Analysis of Herbal Supplement Containing Milk Thistle on Ascentis® Express 2.7 μm C18 application for HPLC

材料

分析柱

产品编号
说明
价格

Ascentis® Express C18, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 3 mm

標準

产品编号
说明
价格

花旗松素

analytical standard

芹甙元-7-葡萄糖苷

analytical standard

槲皮苷

primary reference standard

异水飞蓟宾A

analytical standard

水飞蓟宾B

analytical standard

水飞蓟亭

analytical standard

Silydianin

analytical standard

水飞蓟宾

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)

mobile phase

[A] water with 0.1% formic acid; [B] methanol

gradient

held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min

flow rate

0.6 mL/min

pressure

4105 psi (283 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

20 μL

sample

20 mg/mL in water:ethanol (5:95); sonicate 15 minutes; filter 0.45 μm; dilute to water:ethanol (80:20)

说明

分析報告

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle containing herbal supplement.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany