跳转至内容
Merck
  • Systematic identification of conserved bacterial c-di-AMP receptor proteins.

Systematic identification of conserved bacterial c-di-AMP receptor proteins.

Proceedings of the National Academy of Sciences of the United States of America (2013-05-15)
Rebecca M Corrigan, Ivan Campeotto, Tharshika Jeganathan, Kevin G Roelofs, Vincent T Lee, Angelika Gründling
摘要

Nucleotide signaling molecules are important messengers in key pathways that allow cellular responses to changing environments. Canonical secondary signaling molecules act through specific receptor proteins by direct binding to alter their activity. Cyclic diadenosine monophosphate (c-di-AMP) is an essential signaling molecule in bacteria that has only recently been discovered. Here we report on the identification of four Staphylococcus aureus c-di-AMP receptor proteins that are also widely distributed among other bacteria. Using an affinity pull-down assay we identified the potassium transporter-gating component KtrA as a c-di-AMP receptor protein, and it was further shown that this protein, together with c-di-AMP, enables S. aureus to grow in low potassium conditions. We defined the c-di-AMP binding activity within KtrA to the RCK_C (regulator of conductance of K(+)) domain. This domain is also found in a second S. aureus protein, a predicted cation/proton antiporter, CpaA, which as we show here also directly binds c-di-AMP. Because RCK_C domains are found in proteinaceous channels, transporters, and antiporters from all kingdoms of life, these findings have broad implications for the regulation of different pathways through nucleotide-dependent signaling. Using a genome-wide nucleotide protein interaction screen we further identified the histidine kinase protein KdpD that in many bacteria is also involved in the regulation of potassium transport and a PII-like signal transduction protein, which we renamed PstA, as c-di-AMP binding proteins. With the identification of these widely distributed c-di-AMP receptor proteins we link the c-di-AMP signaling network to a central metabolic process in bacteria.

材料
货号
品牌
产品描述

Sigma-Aldrich
氯化钾, ACS reagent, 99.0-100.5%
Sigma-Aldrich
氯化钾, for molecular biology, ≥99.0%
Sigma-Aldrich
氯化钾, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E508, 99-100.5% (AT), ≤0.0001% Al
Sigma-Aldrich
氯化钾, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%
Sigma-Aldrich
氯化钾, ReagentPlus®, ≥99.0%
Sigma-Aldrich
氯化钾 溶液, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
氯化钾, puriss. p.a., ≥99.5% (AT)
Sigma-Aldrich
氯化钾, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
氯化钾, 99.999% trace metals basis
Supelco
氯化钾 溶液, for Ag/AgCl electrodes, ~3 M KCl, saturated with silver chloride
Sigma-Aldrich
氯化钾, BioXtra, ≥99.0%
Sigma-Aldrich
氯化钾, ≥99.99% trace metals basis
Supelco
氯化钾 溶液, BioUltra, ~3 M in H2O
Supelco
ISA(离子强度调节溶液:1M KCl), 1 M KCl
Sigma-Aldrich
氯化钾 溶液, 0.075 M, sterile-filtered, BioXtra, suitable for cell culture
Supelco
氯化钾 溶液, conductance standard A acc. to ISO 7888, 0.1 M KCl
Sigma-Aldrich
氯化钾, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Supelco
氯化钾 溶液, conductance standard B acc. to ISO 7888, 0.01 M KCl
Sigma-Aldrich
氯化钾, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
氯化钾, tested according to Ph. Eur.
Supelco
氯化钾 溶液, conductance standard C acc. to ISO 7888, 0.001 M KCl