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Merck
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565325-U

Supelco

Ascentis® RP-Amide (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

别名:

RP-Amide反相色谱柱

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1 EA
$1,380.00

$1,380.00

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1 EA
$1,380.00

About This Item

UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

$1,380.00

请联系客服了解存货情况

产品名称

Ascentis® RP-Amide HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

物料

stainless steel column

质量水平

100

Agency

suitable for USP L60

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

标记范围

19.5% Carbon loading

参数

≤70 °C temp. range
400 bar pressure (5801 psi)

技术

HPLC: suitable
LC/MS: suitable

长度 × 内径

25 cm × 4.6 mm

表面积

450 m2/g

表面覆盖度

2.7 μmol/m2

杂质

<5 ppm metals

基质

fully porous particle
silica gel high purity, spherical

基质活性基团

amide, alkyl phase

粒径

5 μm

孔径

100 Å

工作pH范围

2-8

应用

environmental
food and beverages

分离技术

reversed phase

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相关类别

一般描述

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。

Ascentis RP-Amide 反相酰胺柱是新一代超低流失、嵌入极性基团 (EPG) 的键合相，该键合相可提供与常规C18不同的选择性，并提高极性化合物 HPLC 和 LC-MS 分析的分离度。Ascentis RP-Amide 反相酰胺柱是嵌入极性基团 HPLC 键合相的首选。

应用


  • A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: 本研究介绍了一种经过验证的超高效液相色谱 (UHPLC) 方法，该方法采用 RP-Amide 熔融核色谱柱定量测定生咖啡提取物中的咖啡酰奎宁和二咖啡酰奎宁酸。该方法表现出极好的精度、准确度和灵敏度，因而成为分析复杂基质中这些生物活性化合物的宝贵工具。该研究重点介绍了 Ascentis® RP-Amide HPLC 色谱柱在提高分析化学应用中色谱分离度和效率方面的实用性 (Fibigr et al., 2018)。

特点和优势

  • 对极性化合物具有良好的保留性能和峰形
  • 100% 水兼容
  • 超低流失，与 LC-MS 兼容
  • 独特的选择性

推荐产品

发现适用于高效液相色谱或液相色谱-质谱分析的理想LiChropur试剂

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
295054-06
295054-05
295054-04
295054-03
295054-02

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

Questions

1–4 of 4 Questions  

  1. What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?

    1 answer

    1. The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.

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  2. What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column  be stored in?

    1 answer

    1. Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can I use Ascentis Express on a UHPLC system?

    1 answer

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

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