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一般描述
96孔蛋白质沉淀滤板是去除生物血浆/血清中沉淀蛋白质的理想设备。该板由0.2 μm疏水层级过滤器/熔块组成。 首先将生物血浆加入96孔板，然后加入蛋白质沉淀剂（例如乙腈）。经过短暂的混合步骤后，向平板施加真空，过滤器/熔块从样品中去除沉淀的蛋白质。 所得滤液可用于进一步处理和/或分析。
应用
- Determination of estrone sulfate, testosterone, androstenedione, DHEAS, cortisol, cortisone, and 17α-hydroxyprogesterone by LC-MS/MS in children and adolescents.：在儿科人群激素分析中用96孔蛋白沉淀过滤板快速制备样品，显著提高试验通量。这项技术提升激素检测的准确性和效率，有较大的临床和研究意义(Ankarberg-Lindgren et al., 2020)。
- Quantitative Determination of Ethyl Glucuronide and Ethyl Sulfate in Postmortem and Antemortem Whole Blood Using Phospholipid Removal 96-Well Plate and UHPLC-MS-MS.：用96孔过滤板去除血样中的磷脂，更加简便地检测法医和临床毒理学重要物质：乙基葡萄糖醛酸苷和硫酸乙酯(Sidqey et al., 2021)。
- Development of a CDC-certified total testosterone assay for adult and pediatric samples using LC-MS/MS.：用96孔蛋白沉淀过滤板开发美国疾病预防控制中心(CDC)认可的睾酮检测方法，表明本品在提高临床诊断准确性中的重要作用(Star-Weinstock et al., 2019)。
- Validation and application of a liquid chromatography-tandem mass spectrometric method for quantification of the drug transport probe fexofenadine in human plasma using 96-well filter plates.：本文检验了一种分析非索非那定的高通量方法，体现96孔过滤板在临床药理学和生物分析中的增效作用(Stanton et al., 2010)。
其他客户在看
Miau-Guo Kang et al.
Journal of analytical toxicology, 43(2), 96-103 (2018-10-03)
Benzodiazepines is a large drug group used extensively to treat sleep disorders and anxiety; these drugs are frequently associated with various crimes such as murder and drug-facilitated sexual assault. With growing use and misuse of these compounds, confirmatory assays are
商品
An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
实验方案
A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.
相关内容
We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
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What is the Department of Transportation shipping information for this product?
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What Cap Mat is used with 96-Well Protein PP Filter Plate, product 55263-U?
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96 Well Plate, product 55263-U, is a square well design, therefore, use 96 Square Well Piercable Cap Mat 575655-U.
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