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生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
EM09902, recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 116.04 kDa
calculated mol wt 88.05 kDa
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ITGAV(3685)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone EM09902 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects integrin alphaV beta5. It targets an epitope within the extracellular domain.
免疫原
Recombinant fragments corresponding to amino acids 31-987 of integrin alphaV and 24-719 of integrin beta5.
應用
Anti-Integrin alphaV beta5, clone EM09902 ZooMAb, Cat. No. ZRB1191, is a recombinant Rabbit monoclonal antibody that detects Integrin V 5 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin alphaV beta5 in human prostate cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin alphaV beta5 in HT-29 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Integrin alphaV beta5 in one million A549 cells.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin alphaV beta5 in HT-29 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Integrin alphaV beta5 in one million A549 cells.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
標靶描述
Integrin alphaV beta5 (UniProt: P06756/P18084; also known as Fibronectin receptor) are encoded by the ITGAV:ITGB5 genes (Gene ID: 3685/3693) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. Integrin alpha-V, a single-pass membrane protein, heterodimerizes with a beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular region of the alpha subunit. Alpha-V associates with various beta subunits in a non-covalent manner. The structural and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. The alpha-V is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 31-992), a transmembrane domain (aa 993-1016), and a cytoplasmic domain (aa 1017-1048). Integrin beta-5 is synthesized with a signal peptide (aa 1-23), which is cleaved off to produce the mature form that contains an extracellular domain (aa 24-719), a short transmembrane domain (aa 720-742), and a cytoplasmic domain (aa 743-799). Integrin alpha-V beta-5 (ITGAV:ITGB5) serves as a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Clone EM09902 is shown to bind to alpha-V beta-5 with high affinity (EC50 = 5 ng/mL) and it displays low binding to alpha-V beta 3 (EC50 = 7 ug/mL). It is shown to stain all adherent tumor cells that express alphaV beta5. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodman, SL et al., (2012). Biol. Open. 1(4); 329-340).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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