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Merck
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文件

A1595

Sigma-Aldrich

白蛋白 溶液 来源于牛血清

10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

别名:

BSA溶液

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About This Item

CAS号:
9048-46-8
MDL號碼:
MFCD00130384
分類程式碼代碼:
12352202
NACRES:
NA.27

生物源

bovine

無菌

sterile-filtered

產品線

BioReagent

化驗

90-120 mg/mL protein basis (biuret)

形狀

solution

純化經由

heat shock fractionation

包含

does not contain stabilizer or preservative

來源

USA origin

濃度

10% in DPBS

技術

cell culture | mammalian: suitable

雜質

≤0.05% fatty acid
≤200 EU/mL endotoxin

pH值

7.1-7.7

UniProt登錄號

P02769

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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相关类别

應用

建议用作制备确定的脂肪酸补充剂的基础。每摩尔白蛋白最多可装载4摩尔脂肪酸。

BSA是已知的脂肪酸（FA）载体。因此，在与FA相关的应用（如细胞培养）中，特定的BSA可能很重要，因为与控制用于培养细胞系的特定脂肪酸有关；因为不同的细胞系可能对特定的FA;s具有不同的敏感性。因此，不含脂肪酸的BSA可用于细胞培养工作，其中必须仔细控制特定的脂肪酸含量，以便研究人员可以控制其特定细胞系所需的特定FA′s。不含脂肪酸的BSA还可以为研究人员希望在其特定的细胞培养实验中使用的特定脂肪酸提供最佳和最大结合位点。不含脂肪酸BSA的使用也解决了对非无脂肪酸BSA中潜在内源性FA′s的担忧。

特點和優勢

  • 不含脂肪酸/经检测脂肪酸含量低
  • 低内毒素
  • 无菌过滤

準備報告

在DPBS D5652中用热休克分馏的无脂肪酸白蛋白制备。

相關產品

产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Ana Janic et al.
Nature medicine, 24(7), 947-953 (2018-06-13)
It has long been assumed that p53 suppresses tumor development through induction of apoptosis, possibly with contributions by cell cycle arrest and cell senescence1,2. However, combined deficiency in these three processes does not result in spontaneous tumor formation as observed
Surasak Kasetsirikul et al.
The Analyst, 145(23), 7680-7686 (2020-09-26)
This work reports the development of a rapid, simple and inexpensive colorimetric paper-based assay for the detection of the severe acute respiratory symptom coronavirus 2 (SARS-CoV-2) humanized antibody. The paper device was prepared with lamination for easy sample handling and
Véronique Tuloup-Minguez et al.
Autophagy, 7(1), 27-39 (2010-10-29)
Macroautophagy is a major lysosomal degradation pathway for cellular components in eukaryotic cells. Baseline macroautophagy is important for quality control of the cytoplasm in order to avoid the accumulation of cytotoxic products. Its stimulation by various stressful situations, including nutrient
Tapan Dave et al.
SLAS discovery : advancing life sciences R & D, 23(3), 274-282 (2017-11-15)
In the recent past, there has been a growing interest in developing nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) therapeutics. As a result, a need for in vitro cell models of human hepatic steatosis and high-throughput assays to
Guangfeng Geng et al.
eLife, 10 (2021-05-05)
Erythropoietin (EPO) drives erythropoiesis and is secreted mainly by the kidney upon hypoxic or anemic stress. The paucity of EPO production in renal EPO-producing cells (REPs) causes renal anemia, one of the most common complications of chronic nephropathies. Although mitochondrial
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