The XNAT product is a PCR kit optimized for use with tissue samples. None of the components of this kit are formulated with Hanks' Balanced Salt Solution (HBSS).
XNAT
REDExtract-N-Amp™ 组织PCR试剂盒
REDExtract-N-Amp™ Tissue PCR Kit
sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included
别名:
Tissue direct PCR kit, Tissue direct PCR kit with loading dye
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10 REACTIONS
$63.80
100 REACTIONS
$511.00
1000 REACTIONS
$4,680.00
About This Item
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用途
sufficient for 10 reactions
10 reactions sufficient for 10 amplifications
10 reactions sufficient for 10 extractions
sufficient for 100 reactions
100 reactions sufficient for 100 amplifications
100 reactions sufficient for 100 extractions
sufficient for 1000 reactions
1000 reactions sufficient for 1000 amplifications
1000 reactions sufficient for 1000 extractions
品質等級
特點
dNTPs included
hotstart
技術
PCR: suitable
顏色
red
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
REDExtract-N-Amp™植物PCR试剂盒包含从鼠尾和其它动物组织、口腔拭子、毛干和唾液中快速提取和扩增基因组DNA所需的所有试剂。无需机械破碎、有机提取、柱纯化或DNA沉淀。该试剂盒还包括专门配制的热启动PCR ReadyMix™，可直接从提取物中扩增。该配方使用基于抗体的热启动，用于特异性扩增。REDExtract-N-Amp 组织 PCR 试剂盒中含有一种示踪染料，可方便地将PCR反应产物直接上样到琼脂糖凝胶上进行分析。
特點和優勢
- 用于基因分型的快速基因组 DNA 分离的理想选择
- 在 15 分钟内一步提取用于 PCR 的基因组 DNA
- PCR ReadyMix，为提取物直接扩增而专门配制
- 用于基因组 DNA 高特异性 PCR 扩增的热启动抗体
- 提取物在4oC下可稳定保存至少 6 个月。
- REDExtract-N-Amp™含有一种示踪染料，可将PCR反应产物方便地直接上样到琼脂糖凝胶上进行分析。
成分
试剂盒含有Tissue Preparation Solution、Extraction Solution和REDExtract-N-Amp PCR Reaction Mix。
其他說明
REDExtract-N-Amp组织PCR试剂盒为从各种细胞和组织中快速提取DNA以及通过PCR扩增靶标提供了所有必需试剂。 这种新型提取方法无需长时间的酶消化或均质化处理。 该试剂盒还包括特殊配方的热启动PCR反应混合物，可直接从提取物中扩增。 REDExtract-N-Amp PCR ReadyMix含有惰性染料，可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。
原則
Extract-N-Amp 组织PCR试剂盒用于从多种细胞和组织中快速提取DNA并通过PCR反应扩增感兴趣的目标基因。其采用一种新型提取方法，无需进行耗时的酶促降解或均质化反应。该试剂盒同时包含一种专门配制的热启动PCR ReadyMix™，可从提取物直接进行扩增。REDExtract-N-Amp PCR ReadyMix含有可作为示踪染料的惰性染料，使得PCR反应物可以方便地上样到琼脂糖凝胶上进行分析。
该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组提取已在室温下的Tissue Preparation Solution 和Extraction Solution中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和REDExtract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。
该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组提取已在室温下的Tissue Preparation Solution 和Extraction Solution中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和REDExtract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。
该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组DNA提取自在室温下的组织制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和REDExtract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。
法律資訊
凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
相關產品
产品编号
说明
价格
訊號詞
Danger
危險分類
Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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商品
Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
实验方案
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.
exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法，可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。
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What is the composition of the different HBSS (-Ca++, -/+ Mg++)?
1 answer-
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Describe the composition of the different HBSS (-Ca++, -/+ Mg++)
1 answer-
The XNAT product is a PCR kit optimized for use with tissue samples. None of the components of this kit are formulated with Hanks' Balanced Salt Solution (HBSS). See the link below to review the technical bulletin for this kit, which includes a list of components. https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/420/893/h6648for.pdf See the link below to review the formulation table for a number of HBSS products: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/420/893/h6648for.pdf
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Is the extract compatible with SYBR green qPCR?
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The extract from using the XNAT kit can be used for SYBR green qPCR, but we recommend using product S4320, Extract-N-Amp SYBR Green PCR ReadyMix (https://www.sigmaaldrich.com/product/sigma/s4320_. This is a 2X reaction mix containing SYBR Green, buffer, salts, dNTPs, Taq polymerase and JumpStart™ Taq antibody. It is optimized specifically for use with the extraction reagents and contains JumpStart Taq antibody for hot start PCR to enhance specificity and SYBR Green I to act as a nonspecific reporter for real-time PCR. More information on our SYBR Green Extract-N-Amp™ kits can be found here: https://www.sigmaaldrich.com/technical-documents/protocol/genomics/qpcr/sybr-green-extract-n-amp-tissue-pcr-kit
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Are the XNAT, REDExtract-N-Amp® Tissue PCR Kit components available separately?
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Only R4775, the REDExtract-N-Amp PCR Reaction Mix is available separately.
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How do I quantitate my DNA with XNAT, REDExtract-N-Amp® Tissue PCR Kit?
1 answer-
The DNA extract generated using the Extract-N-Amp kit is very crude, and it is not possible to obtain a reliable 260/280 ratio. For quantitation of pure DNA, we recommend using one of our GenElute genomic DNA kits.
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What is the shelf life of the kit XNAT, REDExtract-N-Amp® Tissue PCR Kit and how should I store it?
1 answer-
The shelf life of XNAT has not been determined. However, based on our storage guidelines, it should be usable for 1 year from the date of receipt. The REDExtract-N-Amp Tissue PCR Kit can be stored at 2-8°C for up to three weeks. For long-term storage, greater than three weeks, -20°C is recommended. Do not store in a "frost-free" freezer. We recommend to store in 10 reaction aliquots.
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I am unable to detect any PCR product with Product XNAT, REDExtract-N-Amp® Tissue PCR Kit.
1 answer-
From XNAT Technical Bulletin, troubleshooting guide table: PCR reaction may be inhibited due to contaminants in the tissue extract. Dilute the tissue extract with a 50:50 mix of extraction and neutralization solutions. To test for inhibition, include a DNA control and/or spike a known amount of template (100-500 copies) into the PCR along with the tissue extract.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I store my DNA samples for use with XNAT, REDExtract-N-Amp® Tissue PCR Kit?
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DNA samples can be stored for up to 6 months at 2-8°C with the tissue sample removed.
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