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product name
胰蛋白酶抑制剂 来源于大豆, lyophilized powder
生物源
Glycine max (soybean)
品質等級
種類
Type II-S
形狀
lyophilized powder
分子量
20,100 Da
技術
inhibition assay: suitable
溶解度
balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble
運輸包裝
ambient
儲存溫度
2-8°C
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生化／生理作用
该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X，机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物，然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。
成分
大豆胰蛋白酶抑制剂是在由两个二硫键交联的单个多肽链中含有 181 个氨基酸残基的单体蛋白质。
單位定義
一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A253所需的酶量。
一个胰蛋白酶单位每分钟将产生 0.001 的 ΔA253，BAEE 作为底物，pH 值为 7.6，温度为 25℃；反应体积 3.2 ml，光程为 1 cm。
準備報告
胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊，呈黄色至琥珀色。使用胰蛋白酶消化细胞后，重悬于胰蛋白酶抑制剂溶液中，使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心，得到细胞沉淀。
在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。
在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。
分析報告
1 mg 胰蛋白酶抑制剂，将抑制最低 1.0 mg 胰蛋白酶活性，约 10,000 BAEE 单位/mg 胰蛋白酶。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Chromatograms
application for HPLC-
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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