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Merck

10109878001

Roche

胰蛋白酶抑制剂

from chicken egg white

别名:

胰蛋白酶

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About This Item

分類程式碼代碼:
12352207

生物源

chicken egg white

品質等級

形狀

powder

包裝

pkg of 1 g

製造商/商標名

Roche

濃度

1 mg/mL

技術

cell culture | mammalian: suitable

最適pH

3.8-4.5

溶解度

water: soluble

儲存溫度

2-8°C

一般說明

蛋清胰蛋白酶抑制剂是无血清细胞培养基中胰蛋白酶、纤溶酶和血浆激肽释放酶活性的抑制剂。它不抑制金属、半胱氨酸和天冬氨酸蛋白酶或组织激肽释放酶(丝氨酸蛋白酶)。

應用

胰蛋白酶抑制剂已用于:
  • 作为裂解液组分,用于处理Tsc2fl/fl(对照)和Tsc2fl/flLyz2-Cre(敲除[KO])骨髓来源巨噬细胞(BMDM),以便进行免疫印迹
  • 在脑室区细胞培养物中的干细胞完成消化解离后,灭活胰蛋白酶
  • 在大脑组织完成解离和胰酶消化后,中和胰蛋白酶
  • 终止组织解聚和用于传代培养程序。
鸡胚胰蛋白酶抑制剂用于终止组织解聚和传代培养程序。

準備報告

工作浓度: 0
工作液: 推荐溶剂为蒸馏水。
贮备液:1 mg/mL
储存条件(工作液):-15 至-25℃
在蒸馏水或 1 mM hcl 中至少可稳定 6 个月,当以等分试样冷冻储存时。避免反复冻融循环。

重構

两者均溶于水。推荐的贮备液:1 mg/mL。15°C 至-25°C 条件下等分冷冻储存。至少可稳定储存 6 个月。

儲存和穩定性

2至8°C下储存。(干态保存!)

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine neonates for the isolation of glial subpopulations. The purpose of the procedure is to dissociate microglia, oligodendrocyte progenitor
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The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(49), 9129-9141 (2022-10-22)
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