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T5912

Sigma-Aldrich

Tris 缓冲盐

10X solution

别名:

TBS

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1 L
$323.00

$323.00


预计发货时间2025年4月14日详情


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1 L
$323.00

About This Item

MDL编号:
UNSPSC代码:
12161700
NACRES:
NA.25

$323.00


预计发货时间2025年4月14日详情


获取大包装报价

产品名称

Tris 缓冲盐, 10 ×, solution

无菌性

0.2 μm filtered

表单

solution

浓度

10 ×

储存温度

2-8°C

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应用

Tris缓冲盐水已被用作电极的洗涤缓冲液[1]。它被用于清洗微量滴定板,以供蛋白质免疫印迹实验和筛选方法使用。[2]

其他说明

Western 印迹中的碱性磷酸酶和过氧化物酶偶联物的洗涤缓冲液。工作液含有 20mM Tris(pH 约为 7.4)和 0.9% NaCl

适用性

适合用作斑点杂交印迹分析中碱性磷酸酶或过氧化物酶偶联物的洗涤缓冲液。

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Larry H Stanker et al.
Journal of immunological methods, 336(1), 1-8 (2008-05-03)
Botulinum neurotoxins (BoNT), produced by the anaerobic bacterium Clostridium botulinum, cause severe neuroparalytic disease and are considered the most toxic biological agents known. While botulism is rare in the U.S. it often is fatal if not treated quickly, and recovery
Rapid prototyping of multifunctional microfluidic cartridges for electrochemical biosensing platforms
Kin J
Sensors and Actuators B, Chemical, 202, 60-66 (2014)
Edward Greenfield et al.
eLife, 3 (2014-12-11)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan
Jingtao Zhong et al.
International journal of molecular medicine, 41(5), 2893-2900 (2018-02-14)
Secretory clusterin (sCLU) is overexpressed in cancer and is associated with resistance to chemotherapy in several types of cancer, including hepatocellular carcinoma (HCC). Sorafenib (SOR), a multikinase inhibitor of Raf/mitogen‑activated protein kinase kinase/extracellular signal‑regulated kinase (ERK) signaling and the receptor
Nicholas Wheelhouse et al.
Scientific reports, 6, 37150-37150 (2016-11-15)
Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion

商品

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Questions

1–5 of 5 Questions  
  1. Which is the buffer composition? Thank you!

    1 answer
    1. The composition of this product consists of 20mM Tris and 0.9% NaCl at the 1X concentration.

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  4. I am interested in Tris Buffered Saline solution 10x (T5912) - I know this is sterile filtered but the specification sheet does not state "Sterile" or "pass" for sterility test. Do you provide a sterility test result on this product?

    1 answer
    1. While this material is sterile-filtered as a part of the production process, sterility testing is not performed on the finished product. This product is tested for Appearance, pH, Conductivity, and Dot Blot suitability. Please see the link below to review the product specifications:
      https://www.sigmaaldrich.com/specification-sheets/183/178/T5912-BULK________SIGMA____.pdf

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  5. What is the CAS number for T5912-1L?

    1 answer
    1. Tris Buffered Saline is not assigned a CAS (Chemical Abstract Service) number. This is a multi-component formulation. CAS numbers are assigned to individual compounds only.

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