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推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
H3, monoclonal
表单
buffered aqueous solution
种属反应性
chicken, wide range
包装
antibody small pack of 25 μL
浓度
~2 mg/mL
技术
capture ELISA: suitable
dot blot: 0.5-1 μg/mL
immunocytochemistry: suitable
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
紫外线可诱导形成胸腺嘧啶二聚体，进而引起细胞 DNA 损伤。单克隆抗体与胸腺嘧啶二聚体结合后，可用于研究光致癌过程中的细胞死亡和紫外线诱导 DNA 损伤。抗胸腺嘧啶二聚体单克隆抗体与单链 DNA 中的 (5'-6') 环丁烷型同胸腺嘧啶或胸腺嘧啶胞嘧啶异二聚体结合。该抗体对短寡核苷酸中的二聚体亲和力更低（标记探针需要至少 10-20 个胸腺嘧啶残基的寡核苷酸）。本品有助于借助敏感的非放射性技术分析胸腺嘧啶二聚化。
免疫原
与鸡丙种球蛋白缀合的含四核苷酸的环丁烷胸腺嘧啶二聚体（GTTG）。
应用
单克隆抗胸腺嘧啶二聚体抗体适用于斑点印迹 (0.5-1 mg/mL)、免疫细胞化学(1:100)、以及捕获ELISA。
可以λ0.5-1μg/mL的浓度，通过UV诱导的λ噬菌体DNA片段胸腺嘧啶二聚体进行 Southern 印迹检测。
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
其他说明
经许可分销。
专利WO87/01134，EP 0233 177 B1
专利WO87/01134，EP 0233 177 B1
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途，或人类或动物任何类型的消费或使用。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Tirzah J Weiss et al.
Pigment cell & melanoma research, 36(1), 6-18 (2022-09-24)
Melanin is a free-radical scavenger, antioxidant, and broadband absorber of ultraviolet (UV) radiation which protects the skin from environmental carcinogenesis. However, melanin synthesis and UV-induced reactive melanin species are also implicated in melanocyte genotoxicity. Here, we attempted to reconcile these
Kimberly N Herman et al.
Environmental and molecular mutagenesis, 55(5), 375-384 (2014-02-20)
DNA polymerase η (pol η), of the Y-family, is well known for its in vitro DNA lesion bypass ability. The most well-characterized lesion bypassed by this polymerase is the cyclobutane pyrimidine dimer (CPD) caused by ultraviolet (UV) light. Historically, cellular
Blake Ferguson et al.
eLife, 8 (2019-01-27)
Genetic variation conferring resistance and susceptibility to carcinogen-induced tumorigenesis is frequently studied in mice. We have now turned this idea to melanoma using the collaborative cross (CC), a resource of mouse strains designed to discover genes for complex diseases. We
Detection of cyclobutane thymine dimers in DNA of human cells with monoclonal antibodies raised against a thymine dimer-containing tetranucleotide.
L Roza et al.
Photochemistry and photobiology, 48(5), 627-633 (1988-11-01)
Minimal, superficial DNA damage in human skin from filtered far-ultraviolet C.
R P Hickerson et al.
The British journal of dermatology, 184(6), 1197-1199 (2021-01-17)
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When using PN T1192 as a primary antibody (1/200) on UV irradiated skin samples (IHC, Fresh frozen), I get no signal despite using a fluorescently labelled anti-mouse antibody as detection. The blocking is done with BSA.
1 answer-
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