SRP5127

RhoA，His 标记 人

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 别名: ARH12, ARHA, RHO12, RHOH12
• UNSPSC代码: 12352200
• NACRES: NA.32

属性

• 生物来源: human
• 重组: expressed in E. coli
• 方案: ≥70% (SDS-PAGE)
• 表单: buffered aqueous glycerol solution
• 分子量: ~23 kDa
• NCBI登记号: NM_001664
• 应用: cell analysis
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... RHOA(387)

说明

一般描述

Ras homolog family member A (RhoA) is a small GTPase which is part of the Rho gene family. The gene encoding it is localized on human chromosome 3p21.31.[1][2]

应用

RhoA, His tagged human has been used for in vitro kinase assay.[3]

生化/生理作用

Ras homolog family member A (RhoA) interacts with protein kinases and serves as a target of activated GTPase. RhoA plays an important role in the initial events of cell protrusion. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton. The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signaling using the viral protein F11 and this can enhance the spread of infection. The protein has a role in basal body docking and planar polarization.[1][4][5][6]

外形

Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

制备说明

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

安全信息

• 象形图: GHS08,GHS07
• 警示用语：: Danger
• 危险声明: H315,H319,H360D
• 预防措施声明: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危险分类: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 储存分类代码: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable