价格与库存信息目前不能提供
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生物源
human
重組細胞
expressed in E. coli
化驗
≥70% (SDS-PAGE)
形狀
aqueous solution
分子量
51 kDa
包裝
pkg of 100 μg
濃度
>0.02 mg/mL
技術
cell based assay: suitable
適合性
suitable for molecular biology
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... EIF4E(1977)
一般說明
應用
Suitable substrate for MNK1 kinase.
生化／生理作用
EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.[1][4]
外觀
Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
历史批次信息供参考:
Eukaryotic translation initiation factor 4E (eIF4E) binding site and the middle one-third of eIF4GI constitute the core domain for cap-dependent translation, and the C-terminal one-third functions as a modulatory region.
Morino S, et al.
Molecular and Cellular Biology, 20(2), 468-477 (2000)
Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.
Pyronnet S, et al.
The Embo Journal, 18(1), 270-279 (1999)
Eukaryotic Translation Initiation Factor 4E Is a Feed-Forward Translational Coactivator of Transforming Growth Factor ? Early Protransforming Events in Breast Epithelial Cells.
Decarlo L, et al.
Molecular and Cellular Biology, 35(15), 2597-2609 (2015)
Yao Zhan et al.
The Journal of investigative dermatology, 135(5), 1368-1376 (2015-01-24)
In eukaryotic cells, the rate-limiting component for cap-dependent mRNA translation is the translation initiation factor eIF4E. eIF4E is overexpressed in a variety of human malignancies, but whether it has a role in melanoma remains obscure. We hypothesized that eIF4E promotes
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