SRP0235

PI3 kinase [p110a(H1047R)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• 别名: PIK3CA, Phosphoinositide-3-kinase, catalytic α, PtdIns-3-kinase p110
• UNSPSC代码: 12352200
• NACRES: NA.32

属性

• 生物来源: human
• 重组: expressed in baculovirus infected insect cells
• 方案: ≥60% (SDS-PAGE)
• 表单: aqueous solution
• 比活: ≥1770 pmol/min-μg
• 分子量: 129 kDa (p110α); 88 kDa (p85α)
• 包装: pkg of 20 μg
• 浓度: 0.38 mg/mL
• NCBI登记号: U79143 (p110alpha); XM_043865 (p85alpha)
• UniProt登记号: P42336
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... PIK3CA(5290)

说明

一般描述

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 2