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Merck
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文件

SRE0055

Sigma-Aldrich

蛋白酶抑制剂粉

Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.

别名:

蛋白酶抑制剂混合物粉末

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

bovine lung
yeast

形狀

lyophilized powder

儲存溫度

−20°C

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特異性

抑制丝氨酸、半胱氨酸和金属蛋白酶。

應用

已针对一般用途进行了优化和测试。
蛋白酶抑制剂混合物粉末已用于免疫印迹和免疫沉淀中。

生化／生理作用

该混合物含有单独的组分，包括2mM的AEBSF、0.3 μ的抑肽酶、116 μM的乌苯美司、14 μM的E-64、1 μM的亮肽素和1mM的EDTA。每种组分都具有特定的抑制特性。

AEBSF和抑肽酶可以抑制丝氨酸蛋白酶，包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。乌苯美司可抑制氨肽酶。E-64可抑制半胱氨酸蛋白酶。EDTA是一种金属蛋白酶的抑制剂。亮肽素作用于丝氨酸和半胱氨酸蛋白酶。
蛋白酶有助于将蛋白质分解成可溶性肽和氨基酸。

成分

1瓶血清瓶
组分
AEBSF
抑肽酶
乌苯美司
E-64
EDTA
亮抑酶肽

數量

一瓶可制备100mL混合液，其足以用于抑制约20克细胞提取物中的蛋白酶。
并非所有提取物都含有相同水平的内源酶，并且可能需要调整所需的混合液用量。

準備報告

该产品以冻干粉形式提供。一瓶可制备100毫升混合液。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H315,H319

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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