SHC016V

MISSION^®

Targets no known genes from any species

• 别名: MISSION^®, MISSION^® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
• 分類程式碼代碼: 41106609
• NACRES: NA.51

属性

• 品質等級: 100
• 產品線: MISSION^®
• 濃度: ≥1x10⁶ VP/ml (via p24 assay)
• 運輸包裝: dry ice
• 儲存溫度: −70°C

说明

一般說明

The MISSION^® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION^® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 10⁶ TU/mL via p24 assay.

当使用MISSION^® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。

應用

MISSION^® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.

分析報告

To see more application data, protocols, vector maps visit sigma.com/shrna.

法律資訊

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable