推荐产品
產品線
MISSION®
濃度
≥1x106 VP/ml (via p24 assay)
技術
capture ELISA: 106 TU/mL using p24
運輸包裝
dry ice
儲存溫度
−70°C
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一般說明
The MISSION Luciferase shRNA Control Transduction Particles contain an shRNA sequence targeting Luciferase, Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase.
The Luciferase shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-luciferase shRNA (Prod. No. SHC007). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
The Luciferase shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-luciferase shRNA (Prod. No. SHC007). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
應用
To see more application data, protocols, vector maps visit sigma.com/shrna.
法律資訊
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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