Upon verification, the laboratory has not characterized the biological activity of SAE0089. However, it is known to be reversibly inactivated by oxidation, where the active form is present in a reduced format. Therefore, just prior to application, one could activate it by incubation with DTT for about 15 minutes at 37 degrees Celsius.
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一般描述
Streptolysin O (SLO)是一种免疫原性、氧不稳定的溶血性外毒素，可通过二硫苏糖醇可逆性激活。[1]在大多数A组菌株以及C组和G组链球菌的许多菌株生长过程中，它与包括streptolysin S在内的其他毒素一起释放到细胞外培养基中。[1,2] SLO和Streptolysin S的区别在于SLO具有免疫原性和氧不稳定性，而Streptolysin S具有氧稳定性和非免疫原性，只有与载体蛋白相关时才有活性[3] SLO的溶血活性是通过在含胆固醇脂质膜上形成多聚纳米孔介导的。
SLO 可用于细胞透化或溶血。不同动物种属的红细胞对 SLO 溶血的敏感性差异显著。[1]使用 SLO 对多种细胞类型和各种应用进行了细胞透化。例如，将它用来将反义寡核苷酸导入培养的真核细胞；[3]探讨鸟嘌呤核苷酸类似物对人 T 淋巴细胞磷脂酰肌醇代谢及蛋白激酶 C （PKC）活化的影响；[4]监测膜脂双层内的胆固醇氧化；[5] 并使用外部荧光团标记活细胞内的蛋白质。[6]
SLO 可用于细胞透化或溶血。不同动物种属的红细胞对 SLO 溶血的敏感性差异显著。[1]使用 SLO 对多种细胞类型和各种应用进行了细胞透化。例如，将它用来将反义寡核苷酸导入培养的真核细胞；[3]探讨鸟嘌呤核苷酸类似物对人 T 淋巴细胞磷脂酰肌醇代谢及蛋白激酶 C （PKC）活化的影响；[4]监测膜脂双层内的胆固醇氧化；[5] 并使用外部荧光团标记活细胞内的蛋白质。[6]
应用
透膜允许细胞摄取大分子或带电分子。
生化/生理作用
本品在大肠杆菌中重组表达而成，含有SLO的完整天然蛋白序列(Uniport ID: P0DF96 aa 34-571)，未添加任何纯化标记，计算分子量为60,144 Dalton。材料由含有20 mM钠盐Hepes pH 7.5、150 mM氯化钠和2 mMEDTA的溶液冻干。
能穿透动物细胞膜的巯基活化毒素。
能穿透动物细胞膜的巯基活化毒素。该蛋白作为单体与膜胆固醇结合，随后聚合成围绕>12 nm孔的大弧形和环形结构。
单位定义
一个单位将导致 50 μL 的 2% 人红细胞悬液在 37 °C的磷酸盐缓冲盐水（pH 7.4） 中发生50%溶解。
含 Hepes 缓冲盐和 EDTA的冻干粉。
警示用语：
Danger
危险分类
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Production, purification, and assay of streptolysin O.
J E Alouf et al.
Methods in enzymology, 165, 52-59 (1988-01-01)
Labeling Proteins Inside Living Cells Using External Fluorophores for Fluorescence Microscopy.
Kai Wen Teng et al.
eLife, 6 (2017-02-02)
J D Graves et al.
The Biochemical journal, 265(2), 407-413 (1990-01-15)
A method of membrane permeabilization of T lymphocytes with the bacterial cytotoxin streptolysin O has allowed the effect of guanine nucleotide analogues on phosphatidylinositol metabolism and protein kinase C (PKC) activation to be investigated. The data demonstrate that, in permeabilized
E L Barry et al.
BioTechniques, 15(6), 1016-1018 (1993-12-01)
Cellular uptake of antisense oligonucleotides is critical to their ability to inhibit gene expression. In the present study, phosphodiester oligodeoxynucleotides were introduced into cells during brief permeabilization with the pore-forming agent streptolysin O. The extent of antisense inhibition was dependent
Atsushi Shoji et al.
Journal of pharmaceutical and biomedical analysis, 128, 455-461 (2016-07-01)
Streptolysin O (SLO), which recognizes sterols and forms nanopores in lipid membranes, is proposed as a sensing element for monitoring cholesterol oxidation in a lipid bilayer. The structural requirements of eight sterols for forming nanopores by SLO confirmed that a
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I purchased SAE0089 (streptolysin o). I see in many protocols and one of your datasheets that Streptolysin o should be treated with 10 mM dithiothreitol before using it. However, I don't see that indication anywhere for SAE0089. Do I still need to treat it with DTT?
1 answer-
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