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表单
lyophilized powder
质量水平
比活
≥1,000 units/mg protein
分子量
39.5 kDa
组成
Protein, ≥70% biuret
储存温度
−20°C
基因信息
Escherichia coli CFT073 ... sodA(1040208) , sodB(1036179) , sodC(1036143)
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一般描述
超氧化物歧化酶是存在于植物、动物和微生物的所有需氧细胞中的一组低分子量金属蛋白。它们通过催化超氧自由基阴离子（O2-）发生歧化反应而生成氧和过氧化氢，阻止其破坏性反应。将3-甘油基丙酸自组装在金电极上，可以有效地促进超氧化物歧化酶的直接电子传递。 [1]
应用
生化/生理作用
催化超氧自由基歧化为过氧化氢和分子氧。在防御细胞对抗氧自由基的毒性作用中起到关键作用。与一氧化氮 (NO) 竞争超氧阴离子（与 NO 反应生成过氧亚硝基阴离子），从而 SOD 促进 NO 的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中，SOD 也显示出抑制细胞凋亡的作用。
其他说明
含锰酶
单位定义
在 25℃、pH 7.8 的黄嘌呤氧化酶偶联系统中，1 个单位将抑制细胞色素 c 减少 50%（反应体积 3.0 mL）。黄嘌呤氧化酶浓度应每分钟 产生 0.025±0.005 初始 ΔA 550 。
外形
含 Tris 缓冲盐
分析说明
关于试验方法请参阅 McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969)。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
其他客户在看
M Falconi et al.
Proteins, 47(4), 513-520 (2002-05-10)
Limited proteolysis by trypsin of monomeric Cu,Zn superoxide dismutase from Escherichia coli induces a specific cleavage of the polypeptide chain at the level of Lys60 located in the S-S subloop of loop 6,5 where, when compared to the eukaryotic enzyme
Yoshikazu Kawai et al.
Nature communications, 14(1), 4123-4123 (2023-07-12)
Inhibition of bacterial cell wall synthesis by antibiotics such as β-lactams is thought to cause explosive lysis through loss of cell wall integrity. However, recent studies on a wide range of bacteria have suggested that these antibiotics also perturb central
U Wendling et al.
Journal of immunology (Baltimore, Md. : 1950), 164(5), 2711-2717 (2000-02-29)
Immunization with Mycobacterium tuberculosis heat shock protein (hsp) 60 has been shown to protect rats from experimental arthritis. Previously, the protection-inducing capacity was shown to reside in the evolutionary conserved parts of the molecule. Now we have studied the nature
Anne-Frances Miller et al.
Biochemistry, 44(16), 5969-5981 (2005-04-20)
We report the first spectroscopic observation of substrate analogue binding to the reduced state of iron superoxide dismutase from Escherichia coli (Fe(2+)SOD) and demonstrate that the pH dependence reflects inhibition of anion binding by ionized Tyr34, not loss of a
Vera G Grivennikova et al.
Redox biology, 17, 192-199 (2018-04-28)
The initial rates of superoxide plus hydrogen peroxide (ROS) generation by intact or permeabilized rat heart mitochondria and coupled inside-out bovine heart submitochondrial particles (SMP) oxidizing NAD-dependent substrates, NADH, and succinate were measured by detecting resorufin formation in the Amplex
实验方案
Enzymatic Assay of Superoxide Dismutase
在测定超氧化物歧化酶活性时，使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50％。
Chromatograms
application for HPLCActive Filters
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