推荐产品
生物源
bovine liver
產品線
BioReagent
形狀
powder
比活性
2,000-5,000 units/mg protein
分子量
tetramer ~250 kDa
技術
cell culture | mammalian: suitable
等電點
5.4
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI 密鑰
NUVBSKCKDOMJSU-UHFFFAOYSA-N
基因資訊
cow ... CAT(280743)
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一般說明
CAT（过氧化氢酶）是一种内源性抗氧化酶，因此可保护细胞免受ROS（活性氧）的损害。在人体内，该基因定位至染色体11p13，由12个内含子和13个外显子所组成。
Research Area: Cell Signaling
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
應用
来自牛肝的过氧化氢酶可用于以下应用：
- 凝胶过滤
- Ultrogel和甘油梯度离心上的分析过滤
- 尺寸排阻色谱（HPLC）-雌激素受体的HPLC分析
- 酶检测
- 酒精氧化酶活性
过氧化氢酶作为天然抗氧化剂，可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。 工业上，过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢，并用于检查其对DNA修复大肠杆菌突变体活力的阳性效应。
生化／生理作用
Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
该产品不需要任何的激活剂，但它会受到3-氨基-1-H-1,2,4三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、二硫苏糖醇和硝酸盐的抑制。
注意
过氧化氢酶溶液不应当被冷冻。 冷冻的溶液将会导致50-70%的活性丢失。
單位定義
一个单位的酶将会在25 °C pH7.0是每分钟分解1.0 μ摩尔的H2O2，而H2O2的浓度经A240下降速率测定可知会从10.3下降至9.2 mM。
準備報告
该产品在活性为2,000-5,000 单位/mg是非常适合于细胞培养。 该酶可以1 mg/mL, pH7.0溶于50mM磷酸钾缓冲液中。
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Claire M Doskey et al.
Redox biology, 10, 274-284 (2016-11-12)
Ascorbate (AscH-) functions as a versatile reducing agent. At pharmacological doses (P-AscH-; [plasma AscH-] ≥≈20mM), achievable through intravenous delivery, oxidation of P-AscH- can produce a high flux of H2O2 in tumors. Catalase is the major enzyme for detoxifying high concentrations
G Redeuilh et al.
The Journal of biological chemistry, 262(15), 6969-6975 (1987-05-25)
The structure of the calf uterus nontransformed molybdate-stabilized estradiol receptor (ER) has been investigated using affinity labeling with tamoxifen aziridine and several monoclonal antibodies directed either against the steroid binding protein (Mr approximately 65,000) or against the heat shock protein
Yanqiong Liu et al.
Medicine, 94(13), e702-e702 (2015-04-04)
Reactive oxygen species (ROS) play critical roles in hepatocarcinogenesis. The catalase (CAT) enzyme is involved in the repair of ROS. Therefore, we investigate the association between CAT gene polymorphisms and the risk of hepatocellular carcinoma (HCC). A total of 715
Theppanya Charoenrat et al.
Bioprocess and biosystems engineering, 27(6), 399-406 (2005-08-05)
An oxygen-limited fed-batch technique (OLFB) was compared to traditional methanol-limited fed-batch technique (MLFB) for the production of recombinant Thai Rosewood beta-glucosidase with Pichia pastoris. The degree of energy limitation, expressed as the relative rate of respiration (q(O)/q(O,max)), was kept similar
Taku Amo et al.
Journal of bacteriology, 184(12), 3305-3312 (2002-05-25)
We had previously isolated a facultatively anaerobic hyperthermophilic archaeon, Pyrobaculum calidifontis strain VA1. Here, we found that strain VA1, when grown under aerobic conditions, harbors high catalase activity. The catalase was purified 91-fold from crude extracts and displayed a specific
实验方案
This procedure may be used for all Catalase products.
过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。
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