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Sigma-Aldrich

凝血酶 CleanCleave™ 试剂盒

别名:

Thrombin Cleavage Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.56

形狀

suspension

品質等級

200

分子量

37 kDa

技術

protein purification: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

凝血酶 CleanCleave 试剂盒包含通过固定牛凝血酶产生的 50％ 凝血酶 - 琼脂糖悬浮液，用于切割重组融合蛋白

應用

用于从含有凝血酶识别序列的重组融合蛋白切割标签。凝血酶 - 琼脂糖与在各种类型的表达系统中表达的重组蛋白相容。

特點和優勢

  • 在短短 2 小时内快速、有效地裂解
  • 凝血酶与琼脂糖共价结合，易于除去。
  • 在 4°C 到 37°C 的温度范围内，以及在广泛的 pH 和离子强度范围内，强裂解反应是有效的。
  • 即使在 0.1％Triton™，1M 尿素或 5mM EDTA 存在下，也能裂解标签
  • 凝血酶 - 琼脂糖是可重复使用的

數量

200 μl 50％ 的凝血酶 - 琼脂糖浆液裂解 >85% 的 1mg 融合蛋白。

法律資訊

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

仅试剂盒组分

产品编号
说明
  • Thrombin Cleavage Buffer, 10× 10 mL

相關產品

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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凝血酶 来源于牛血浆 ≥60 NIH units/mg protein (biuret)

Sigma-Aldrich

T7326

凝血酶 来源于牛血浆

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