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RAB0480

Sigma-Aldrich

大鼠肿瘤坏死因子α ELISA试剂盒

for cell and tissue lysates

别名:

TNF-α

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1 KIT
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1 KIT
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About This Item

UNSPSC代码:
41116158
NACRES:
NA.32

$810.00

请联系客服了解存货情况

种属反应性

rat

包装

kit of 96 wells (12 strips x 8 wells)

技术

ELISA: suitable
capture ELISA: suitable

输入

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

检测方法

colorimetric

运输

wet ice

储存温度

−20°C

基因信息

rat ... Tnf(24835)

相关类别

一般描述

大鼠 TNF-α ELISA（酶联免疫吸附测定）试剂盒是定量测定细胞裂解液和组织裂解液中大鼠 TNF-α 的一种体外酶联免疫测定法。

免疫原

重组大鼠 TNF-α

应用

仅限研究用途，不可用于诊断。
大鼠肿瘤坏死因子 α ELISA 试剂盒已用于测定细胞裂解液和组织裂解物中的大鼠肿瘤坏死-α（TNF-α）的浓度。[1][2]

生化/生理作用

肿瘤坏死因子-α（TNF-α）影响脑损伤后的血脑屏障、炎症、血栓形成和血管变化等各种细胞过程。TNF-α 的表达在阿尔茨海默病、多发性硬化症、帕金森氏病、脑膜炎球菌性脑膜炎和 HIV 感染等各种中枢神经系统疾病中上调。脑和血液中 TNF-α 表达上调（响应脂多糖）可能会使得高血压大鼠的脑干血栓形成和出血以及中风敏感性/风险增加。[3]肿瘤坏死因子-α 激活多形核白细胞 NADPH 氧化酶，从而促进大鼠全身性氧化应激（OS）、炎症和高血压的发生。[4]

其他说明

本产品提供样本分析证书。
请在批号对应的文本框中输入单词 sample 。

试剂盒组分也可单独购买

产品编号
说明
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  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)化学品安全说明书
  • RABLYSIS12X Cell Lysis Buffer (Item J)化学品安全说明书
  • RABSTOP3ELISA Stop Solution (Item I)化学品安全说明书
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)化学品安全说明书
  • RABWASH420X Wash Buffer (Item B)化学品安全说明书

象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290

预防措施声明

P234 - P390

危险分类

Met. Corr. 1

储存分类代码

8A - Combustible corrosive hazardous materials

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0602D0715
0424K0715
0404K0715
0320K0715
1114J0715

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Nanik Sundari et al.
Journal of toxicology, 2018, 2642714-2642714 (2019-01-12)
Phaleria macrocarpa is one of the Indonesian herbal plants which has been shown to have a hepatoprotective effect. This study was conducted to evaluate the protective effect of water extract of mahkota dewa (Phaleria macrocarpa) in liver fibrosis and to
Vu Thi Thu et al.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 20(3), 305-314 (2016-05-11)
Inflammatory and fibrotic responses are accelerated during the reperfusion period, and excessive fibrosis and inflammation contribute to cardiac malfunction. NecroX compounds have been shown to protect the liver and heart from ischemia-reperfusion injury. The aim of this study was to
R A Ajala-Lawal et al.
Archives of physiology and biochemistry, 126(2), 107-115 (2018-10-06)
This study investigated the influence of betulinic acid on high-fructose diet-induced metabolic syndrome in rats. Oral administration of betulinic acid significantly reversed high-fructose diet-mediated increase in body mass index and blood glucose. Furthermore, betulinic acid restored high-fructose diet-mediated alterations in
Meijuan Chen et al.
Gastroenterology research and practice, 2019, 8723460-8723460 (2019-10-01)
Phosphatidylcholine (PC) is the major surface-active phospholipid and creates a hydrophobic nature to the surface. It has been reported to reverse the progression of liver fibrosis and to improve liver function. The aim of the present study was to evaluate
Tumor necrosis factor-alpha: a possible priming agent for the polymorphonuclear leukocyte-reduced nicotinamide-adenine dinucleotide phosphate oxidase in hypertension.
Mazor R
Hypertension, 55(2), 353-362 (2010)
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Questions

  1. how many tests can be done by a kit

    1 answer

    1. The Rat TNF-alpha ELISA kit provides one 96-well plate. A set of 7 standards is loaded in duplicates to generate a standard curve, leaving 82 wells for testing 41 samples in duplicates.

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