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R6625

Sigma-Aldrich

核糖核酸 来源于圆酵母

Type VI

别名:

核糖核酸

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25 G
$199.00
100 G
$674.00
1 KG
$3,240.00

$199.00


预计发货时间2025年1月31日详情


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25 G
$199.00
100 G
$674.00
1 KG
$3,240.00

About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
41106305
NACRES:
NA.51

$199.00


预计发货时间2025年1月31日详情


获取大包装报价

種類

Type VI

品質等級

儲存溫度

−20°C

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一般說明

来自圆酵母的核糖核酸(RNA)是RNase酶的底物。 从酵母中分离RNA是一个复杂的过程,涉及在苯和洗涤剂(SDS)环境中对细胞进行加热和冷冻循环。[1]

應用

核糖核酸(RNA)适用于Northern印迹杂交、逆转录聚合酶链式反应(RTPCR)和cDNA构建等应用。[1]

来自酵母的核糖核酸(RNA)可用作研究酶如核糖核酸酶-A、核糖核酸酶 T1(RNAase)和三角梅抗病毒蛋白 1(BBAP1)的核糖核酸酶活性的底物

品質

磷:8.0-10.0%(无水)

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Slide 1 of 1

1 of 1

Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Stephanie L Grillo et al.
Purinergic signalling, 15(3), 327-342 (2019-07-06)
Adenosine is an endogenous nucleoside in the central nervous system that acts on adenosine receptors. These are G protein-coupled receptors that have four known subtypes: A1, A2A, A2B, and A3 receptors. In the present study, we aimed to map the
Jay S Petrick et al.
Regulatory toxicology and pharmacology : RTP, 81, 57-68 (2016-10-30)
Genetically modified (GM) crops have been developed and commercialized that utilize double stranded RNAs (dsRNA) to suppress a target gene(s), producing virus resistance, nutritional and quality traits. MON 87411 is a GM maize variety that leverages dsRNAs to selectively control
Shahab M Danesh et al.
Gene expression patterns : GEP, 9(5), 255-265 (2009-04-28)
Cell-cell communication is critical for regulating embryonic organ growth and differentiation. The Bone Morphogenetic Protein (BMP) family of transforming growth factor beta (TGFbeta) molecules represents one class of such cell-cell signaling molecules that regulate the morphogenesis of several organs. Due
Jesus E Martinez-Lopez et al.
Frontiers in neuroanatomy, 9, 12-12 (2015-03-06)
In the study of central nervous system morphogenesis, the identification of new molecular markers allows us to identify domains along the antero-posterior and dorso-ventral (DV) axes. In the past years, the alar and basal plates of the midbrain have been

Questions

1–8 of 8 Questions  
  1. What are the nucleotide sequences of the mixture of RNA? 

    1 answer
    1. This product is a heterogeneous mixture of tRNA molecules This material has not been sequenced.

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  2. How many base pairs are in the RNA? Can I dossolve it in TE buffer?

    1 answer
    1. This product is a heterogeneous mixture of RNA molecules ranging from 3000 to 40000 daltons which corresponds to approximately 10 to 120 base pairs in size. The material is tested for solubility at 10 mg per 1 ml of 0.1M Acetate buffer, pH 5.0. Testing is TE buffer is not performed. However, this buffer is commonly used as short term storage buffer for RNA.

      Please see the link below to review the oligo handling and stability guide for additional information: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/dna-and-rna-purification/oligonucleotide-handling-and-stability

      Helpful?

  3. I would like to know if this product RNA from torula yeast (R6625) contains only mRNA and rRNA or does it also contains transfer-RNA?

    1 answer
    1. This product is not assayed for the distribution of RNA forms, but it is expected to be a heterogeneous mixture ranging from 3000 to 40000 Daltons.

      Helpful?

  4. What does type VI mean?

    1 answer
    1. The 'type' designation for this product is an internal definition of Sigma and has no specific biological significance. It is in reference to the method of manufacture or product preparation.

      Helpful?

  5. What does Type IV refer to?

    1 answer
    1. The 'type' designation for this product is an internal definition of Sigma and has no specific biological significance. It is in reference to the method of manufacture or product preparation.

      Helpful?

  6. What is the molecular weight of this product? What is the RNA concentration?

    1 answer
    1. This product is a heterogeneous mixture of RNA molecules ranging from 3000 to 40000 in MW. The material is not tested for purity. There is a maximum allowable water content specification of 10%. Please see the link below for review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/R6625-BULKWXBF1071V.pdf

      Helpful?

  7. What species is Product R6625, Ribonucleic acid from torula yeast, from?

    1 answer
    1. The former taxonomic name for Torula yeast is Torulopsis utilus; the current name is Candida utilus.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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