For testing purposes, this product is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. The solution stability and storage conditions have not been determined. The enzyme may also be dissolved in 0.11 M Tris HCl buffer, pH 8.9 with 0.11 M NaCl and 15 mM MgCl2 according to Worthington Manual, p. 310 (1993). Note that this material is not the raw venom, but the purified enzyme. The enzyme is purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.
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应用
磷酸二酯酶（PDE）是一种可用于破坏磷酸二酯键的酶。 它是一种膜结合糖蛋白，可用于催化各种核苷酸多磷酸盐的水解。 磷酸二酯酶I可用于磷酸二酯酶活化检测实验中以水解AMP。 产品P3243来自于 响尾蛇（Crotalus adamanteus） 的毒液中，并已经过纯化。 产物P3243已被用于将含有怀俄苷衍生物的tRNA水解成单核苷[1]。
生化/生理作用
磷酸二酯酶I可破坏磷酸二酯键并催化各种核苷酸多磷酸盐的水解。 通过磷脂酰肌醇特异性磷脂酶C从真核质膜中释放磷酸二酯酶I。
单位定义
在25°C、pH 8.9条件下，1单位每分钟可水解1 μmol对硝基苯基胸苷5磷酸酯
制备说明
通过Williams等人[2] 的方法进行纯化，并进一步处理以灭活5′-核苷酸酶活性污染。
重悬
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Action of venom phosphodiesterase on deoxyribonucleic acid.
E J WILLIAMS et al.
The Journal of biological chemistry, 236, 1130-1134 (1961-04-01)
Valérie de Crécy-Lagard et al.
Molecular biology and evolution, 27(9), 2062-2077 (2010-04-13)
Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNA(Phe)), 3' adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a
Jongwon Lim et al.
ACS omega, 5(23), 14173-14179 (2020-06-23)
The metazoan second messenger 2'3'-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) is a cyclic dinucleotide (CDN) that induces secretion of type I interferons and activates the immune system and has thus attracted significant interest as a vaccine adjuvant or immunotherapeutic. CDN bisphosphorothioates
Alain R Weber et al.
Nature communications, 7, 10806-10806 (2016-03-05)
Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface only recently with the discovery of the 5-methylcytosine (5mC)-directed hydroxylase and base
N Shenoy et al.
Blood cancer journal, 7(7), e587-e587 (2017-07-22)
The Ten Eleven Translocation (TET) enzymes have been found to be mutated in both diffuse large B-cell (DLBCL) and peripheral T-cell (PTCL) lymphomas resulting in DNA hypermethylation. Recent studies in embryonal stem cells showed that ascorbic acid (AA) is a
实验方案
在测定5′-核苷酸酶活性时，本操作流程使用5′-单磷酸腺苷和一种显色试剂生成的标准曲线而对释放的磷的微摩尔数进行测定。
To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.
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1. If I want to dissolve this venom what I can use distilled water or any buffer? And what is the storage temperature after reconstitution? 2. If I want to increase the efficiency of venom what I can use?
1 answer-
Helpful?
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What buffer can be used to Store PDE I, and is it soluble in water?
1 answer-
For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL. The solution stability has not been determined.
Helpful?
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