It is crucial to adhere to the instructions for the Reticulocyte Stain Protocol (R4132) precisely. 10 minutes means 10 minutes. If you exceed 10 minutes, the test results may be inaccurate. The expectation is that 10 minutes was chosen to minimize the number of false positive results. Incubating the slides longer than 10 minutes is not recommended.
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表单
solution
保质期
Expiry date on the label.
IVD
for in vitro diagnostic use
技术
microbe id | staining: suitable
应用
hematology
histology
储存温度
room temp
应用
Utilizes New Methylene Blue for the identification of reticulocytes in blood films. Reticulocyte Stain has been used in the detection of reticulocytes.[1]
原理
Blood and reticulocyte staining solution (New Methylene Blue) are mixed and incubated briefly at room temperature. Wedge or spun smears are made on microscope slides, air dried and evaluated under oil immersion on a light microscope. A red blood cell scoring positive would be observed containing two or more blue-stained granules.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Can incubation time be longer than the 10 minutes specified in the Instructions For Use? If so, for how long? Also, in general, how much time can lapse between step 1 and step 2?
1 answer-
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