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等級
for molecular biology
形狀
buffered aqueous glycerol solution
濃度
≥5000 units/mL
5,000 units/mL
運輸包裝
wet ice
儲存溫度
−20°C
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特異性
Recognition sequence: 5′-CGAT/CG-3′
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
應用
PvuI is a restriction endonuclease that is used for molecular biological applications to cleave DNA at the recognition sequence 5′-CGAT/CG-3′ to generate fragments with 3′-cohesive termini.
其他說明
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
外觀
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 300 mM KCl, 1 mM dithioerythritol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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