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用途
sufficient for 350 purifications
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技術
DNA purification: suitable
環保替代類別
儲存溫度
15-25°C
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一般說明
GenElute 质粒小量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒结合了硅胶结合技术和离心柱形式的便利性，可在30分钟内从1-5 mL大肠杆菌培养物中回收出至多15 μg高拷贝数质粒DNA。请注意，实际得率和最佳的培养物体积取决于质粒和培养基。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后，在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。随后通过离心洗涤步骤去除污染物。最后，在水或Tris-EDTA缓冲液中洗脱结合所得的DNA。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，本品是一种本安型化学制剂。
應用
此类DNA可直接用于限制性酶切、克隆、PCR、转化、转录、传统和自动化测序等下游应用。
该试剂盒已用于匐行恶丝虫质粒DNA的纯化。
特點和優勢
- 可在30分钟内获得纯化的质粒DNA
- 至多可纯化出15 μg质粒DNA
- 检测不到基因组DNA或RNA污染
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
其他說明
更多信息，请见www.sigma-aldrich.com/genelutehp。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
3 - Flammable liquids
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