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一般說明
GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。
應用
GenElute™ PCR产物纯化试剂盒已被用于从反应的其他组分中快速纯化单链或双链PCR扩增产物（100bp至10kb）。
纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。
特點和優勢
- 可在8分钟内纯化出多达100 μl或10 μg 的PCR扩增产物DNA。
- 可回收95%长度介于100 bp至10 kb之间的PCR产物
- 可去除99%以上的引物和其他成分
- 不需要去除有机萃取引入的矿物油
- 相对于其他市场领先竞争对手，可多获得40%的纯化制备物
其他說明
GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。
原則
GenElute PCR回收试剂盒将硅胶结合和微量离心形式的优势结合在一起。DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后，再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA，至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(可去除<40 bp的片段)。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Genetic identification of intestinal microsporidia species in immunocompromised patients in Tunisia.
Najla Chabchoub et al.
The American journal of tropical medicine and hygiene, 80(1), 24-27 (2009-01-15)
Stool samples from 86 immunocompromised patients (51 human immunodeficiency virus (HIV)-infected patients and 35 patients with haematologic malignancies) were systematically screened for intestinal microspordiosis by microscopic examination and polymerase chain reaction (PCR) using universal primer V1/PMP2. Nine samples (10.5%) showed
Charlotte G Cole et al.
Genome biology, 9(5), R78-R78 (2008-05-15)
Although the human genome sequence was declared complete in 2004, the sequence was interrupted by 341 gaps of which 308 lay in an estimated approximately 28 Mb of euchromatin. While these gaps constitute only approximately 1% of the sequence, knowledge
Genetic analysis of uveal melanoma by array comparative genomic hybridization before and after radiotherapy
Werner Wackernagel
Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry, 27.6, 286-291 (2013)
Andreas Veith et al.
Frontiers in microbiology, 2, 37-37 (2011-07-13)
The sulfur oxygenase reductase (SOR) is the initial enzyme of the sulfur oxidation pathway in the thermoacidophilic Archaeon Acidianus ambivalens. The SOR catalyzes an oxygen-dependent sulfur disproportionation to H(2)S, sulfite and thiosulfate. The spherical, hollow, cytoplasmic enzyme is composed of
Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization.
Vandewoestyne M
International Journal of Cancer. Journal International Du Cancer, 130(5), 1098-1108 (2012)
实验方案
GenElute™ PCR Clean-up Kit rapidly purifies PCR products from excess components, ensuring clean samples for downstream applications.
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
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