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Merck

P8240

Sigma-Aldrich

REDExtract-N-Amp PCR ReadyMix for Blood

12 mL sufficient for 1000 amplifications

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.55

品質等級

用途

sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications

特點

dNTPs included
hotstart

顏色

red

運輸包裝

wet ice

儲存溫度

−20°C

相关类别

一般說明

REDExtract-N-Amp PCR ReadyMix for Blood is a PCR master mix containing buffer, salts, dNTPs, and Taq polymerase. This 2x reaction mixture also contains JumpStart Taq antibody for specific hot start amplification and REDTaq® dye, an inert tracking dye to enable direct loading of PCR products onto an agarose gel for analysis. PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Blood PCR Kits. All Extract-N-Amp Kit include a volume of PCR ReadyMix sufficient for at least one PCR reaction per extraction. However, where additional amplifications are required, supplemental PCR ReadyMix may be needed.

應用

REDExtract-N-Amp PCR ReadyMix for Blood has been used for polymerase chain reaction (PCR).

特點和優勢

  • Additional loading dye is not required as the PCR reaction mix contains REDTaq® dye for direct and convenient loading onto an agarose gel for analysis
  • Hotstart feature allows for room temperature set-up without premature Taq DNA polymerase activity
  • Ready-mix enables time-saving procedures
  • Reduced risk of contamination from multiple pipet steps and increased reproducibility

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Sofia Lejon Crottet et al.
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 50(2), 169-174 (2014-04-01)
Starting in 2013, blood donors must be tested at least using: (1) one monoclonal anti-D and one anti-CDE (alternatively full RhCcEe phenotyping), and (2) all RhD negative donors must be tested for RHD exons 5 and 10 plus one further

实验方案

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。

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