The concentration of p-nitrophenylphosphate (pNPP) in this product is 1.5 mg/mL. This item is a ready-to-use product and dilution is not recommended. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/285/284/p7998dat.pdf
$187.00
国内现货，预计发货时间2025年4月09日详情
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一般描述
本品是由 对硝基苯基磷酸酯（pNPP）组成的缓冲碱性磷酸酶底物。这个先进的基质系统含有不溶性稳定剂，可提高稳定性和反应性，并降低基线吸收。它产生一种可溶性终产物，因此不推荐用于蛋白质印迹或组织化学应用。
应用
产生一种可溶性黄色反应产物，可在405nm处读取。对于终点测定，可以使用3N NaOH终止反应。推荐用于ELISA（微孔）分析；不建议用于膜应用。
注意
可在2-8°C下稳定保存至少一年。
外形
即用型
制备说明
含有对硝基苯基磷酸酯，它是碱性磷酸酶的底物。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Calcium pyrophosphate deposition (CPPD) is associated with osteoarthritis and is the cause of a common inflammatory articular disease. Ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (eNPP1) is the major ecto-pyrophosphatase in chondrocytes and cartilage-derived matrix vesicles (MVs). Thus, eNPP1 is a principle contributor to
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Hi, may I know what is the concentration of this solution? Because I need to do the calibration curve. Thank you
1 answer-
Helpful?
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Can this be used after it has been frozen?
1 answer-
The stability of this solution has not been evaluated at −20°C. The freeze/thaw process may contribute to decomposition of the substrate solution. Signs of degradation include changes in color or turbidity and precipitation of the pNPP.
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