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10 MG
$473.00
100 MG
$2,370.00
About This Item
$473.00
请联系客服了解存货情况
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表单
powder
质量水平
比活
≤0.02 units/mg protein (biuret)
杂质
salt, free
溶解性
0.85% sodium chloride: soluble 15 mg/mL, clear to slightly hazy, colorless to light yellow
储存温度
2-8°C
生化/生理作用
HRP易与过氧化氢（H2O2）结合，所得[HRP-H2O2]配合物可氧化各种氢供体。最佳pH为6.0-6.5，酶在5.0-9.0的pH范围内最稳定。HRP可以通过几种不同的方法与抗体结合，包括使用戊二醛、高碘酸盐氧化、二硫键以及氨基和巯基定向交联剂。它比酶标签，β-半乳糖苷酶和碱性磷酸酶更小、更稳定。因此，它是最理想的标签。而且，糖基化使其非特异性结合较低。[4]它还用于测定溶液中的葡萄糖[5] 和过氧化物[6] 。已发现叠氮化钠、氰化物、L-胱氨酸、重铬酸盐、亚乙基硫脲、羟胺、硫化物、钒酸盐、对氨基苯甲酸、以及Cd2+、Co2+、Cu2+、Fe3+、Mn2+、Ni2+、铅2+ 等离子可抑制其酶活性。 [7]
灭活的过氧化物酶可与山羊和兔子的抗过氧化物酶发生反应。 它可以通过多种方法被重新激活，例如加热[1]。
单位定义
在20°C、pH 6.0条件下，一单位可在20秒内从邻苯三酚形成1.0 mg的红棓酚。
制备说明
通过对Teale方法的改进，由VI型辣根过氧化物酶进行制备。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
S Morimoto et al.
The Journal of biological chemistry, 274(37), 26192-26198 (1999-09-03)
Elicitor treatment or mechanical damage to Scutellaria baicalensis Georgi (skullcap plants) callus causes an immediate insolubilization of a 36-kDa protein into cell walls. The 36-kDa protein was identified as peroxidase 1 by analysis of its internal amino acid sequence and
L L Vacca et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(3), 208-215 (1975-03-01)
Immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. Compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear
Dual projections of single neurons are visualized simultaneously: use of enzymatically inactive [3H]HRP.
N L Hayes et al.
Brain research, 165(2), 321-326 (1979-04-13)
C J Forehand et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2(5), 654-652 (1982-05-01)
This study of spinal cord injury in bullfrog (Rana catesbeiana) tadpoles using the neuroanatomical tracer horseradish peroxidase (HRP) was undertaken to determine (1) whether the same anatomical regions that normally give rise to ascending or descending spinal tracts do so
Cleavage of the haem-protein link by acid methylethylketone.
F W TEALE
Biochimica et biophysica acta, 35, 543-543 (1959-10-01)
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