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生物源
rabbit muscle
形狀
lyophilized powder
比活性
20-30 units/mg protein
純化經由
2× crystallization
成份
Protein, 30-60% E1%/280
異物活動
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%
儲存溫度
−20°C
一般說明
磷酸化酶 A 是糖原磷酸化酶的活性形式,其将糖原和正磷酸盐(Pi)转化为葡萄糖1-磷酸(G-1-P)。
應用
来自兔肌肉的磷酸化酶已用于混合糖原以制备极限糊精。它还被用于探测寡聚糖微阵列磷酸化酶活性分析中的阵列。
来自兔肌肉的磷酸化酶已被用于研究中评估齐墩果酸的分子机制。它也被用于研究中描述在 32P-ATP 存在下磷酸化酶 B 通过转化酶催化转化为磷酸化酶 A 的过程。
生化/生理作用
磷酸化酶 A 可以被以下化合物抑制:多氯联苯、多氯联苯和多溴联苯。
磷酸化酶参与糖原分解。磷酸化酶缺乏导致多种糖原贮积病和肝肿大。
通过磷酸化酶激酶的作用,二聚磷酸化酶 b 被转化为更有活性的四聚体磷酸化酶 a。
單位定義
在含有磷酸葡萄糖苷酶,NADP 和葡萄糖-6-磷酸脱氢酶的系统中测量,一个单位的酶在 pH 6.8,30℃ 下每分钟将糖原和正磷酸盐转化为 1.0 μmole α-D-1 葡萄糖 1-磷酸酯。(一个 μmolar 单位相当于约 45 Cori 单位。)
外觀
冻干粉末,含有 β-甘油磷酸盐和 EDTA
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Hypoglycemia in the toddler and child
Pediatric Endocrinology, Diabetes, and Metabolism, 920-955 (2014)
Activity of porcine muscle glycogen debranching enzyme in relation to pH and temperature
Meat Science, 69(1), 143-149 (2005)
Glycogen Phosphorylase Structures and Function
Accounts of Chemical Research, 15, 361-369 (1982)
The Plant cell, 16(3), 694-708 (2004-02-20)
Protein phosphorylation in amyloplasts and chloroplasts of Triticum aestivum (wheat) was investigated after the incubation of intact plastids with gamma-(32)P-ATP. Among the soluble phosphoproteins detected in plastids, three forms of starch branching enzyme (SBE) were phosphorylated in amyloplasts (SBEI, SBEIIa
Biochimica et biophysica acta, 701(2), 173-179 (1982-02-18)
Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls inhibited both rabbit muscle phosphorylase a and phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-d-glucosyltransferase, EC 2.4.1.1). The degree of inhibition was dependent upon the relative hydrophobicity of the compounds and steric hinderance. 2,4,5,2',4',5'-Hexabromobiphenyl and Firemaster BP-6
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