The protocol for cell samples using MAK434 is as follows:
- Suspend about two million (2 × 10^6) harvested cells in 400 μL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge the homogenate at 14,000 g for 10 min and transfer the clear supernatant into a clean tube. It is advisable to run a pilot test of the sample at different dilutions and select a dilution with readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
- If a lysis buffer is to be used, it is recommended to first run an assay to test if components in the lysis buffer interfere with the assay. This can be accomplished by running two standard curves: one prepared in H2O and one prepared in the same proportion of lysis buffer that the sample will contain. If the two standard curves are the same, there is no interference and it is safe to use the lysis buffer. If there is a difference in the standard curves, the lysis buffer may still be used if the standard curve remains linear; however, the standard curve to be used for analyzing the lysate samples will need to be prepared in the lysis buffer.
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质量水平
输入
cell culture medium
tissue
serum
urine
food(s)
beverage(s)
plasma
应用
agriculture
cosmetics
environmental
food and beverages
检测方法
colorimetric
相关疾病
gastrointestinal diseases
储存温度
−20°C
一般描述
Acetaldehyde (CH₃CHO) is a commonly found aldehyde in nature and widely used in various industries. It is a metabolic byproduct of ethanol in the liver and is toxic to the human body. However, it is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. Individuals deficient in aldehyde dehydrogenase experience accumulation of acetaldehyde upon alcohol consumption, leading to facial and body flushing known as alcohol flush reaction or “Asian flush syndrome.” Acetaldehyde buildup has also been linked to alcohol-related hangover effects. Despite its classification as a carcinogen, acetaldehyde is naturally present in many foods and beverages such as ripe fruit, coffee, and wine.
应用
The Acetaldehyde Assay Kit may be used for:
- Alcohol Metabolism Research
- Hepatology Research
- Neurological Research
特点和优势
- Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent and only a 30 minute assay room temperature reaction, saving you time and effort.
- Convenient and Sensitive: Uses only 20 µL of sample for the determination of acetaldehyde with a detection range of 2 µM to 2mM.
- High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, ensuring efficiency and accuracy.
适用性
The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).
原理
The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.
其他说明
For additional information on our range of Biochemicals, please complete this form.
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
12 - Non Combustible Liquids
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
-
How should MAK434 be used for cell samples?
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