Acidification of urine samples is necessary for longer-term storage. Oxalate demonstrates greater solubility under acidic conditions. Additionally, ascorbic acid (Vitamin C) in urine samples rapidly degrades into oxalate in non-acidified samples, so acidifying urine should help to prevent this. Given that the Working Reagent used in this assay is acidic, acidifying fresh samples prior to the assay should not be necessary.
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檢測方法
colorimetric
相關疾病
genitourinary disorders
儲存溫度
−20°C
一般說明
在真核生物的三羧酸循环和其他微生物的乙醛酸循环中，草酸盐或草酸是一种代谢分解产物。草酸盐或草酸常见于人和其他哺乳动物的尿液中。草酸浓度可用于检测肾功能，高水平草酸指示存在主要由不溶性草酸钙盐形成的肾结石。作为肾结石的标记物，检测草酸钙比检测钙浓度更准确，因为即使是在正常尿液中，钙的分泌浓度页较高。
特點和優勢
与高通量处理系统兼容。
適合性
适用于尿液、动植物组织样品中草酸盐（草酸）的定量测定。
原則
简单、直接和高通量的测定草酸浓度的方法有着广泛的应用。该草酸盐检测试剂盒使用单一工作试剂，将草酸氧化酶反应和显色反应一步结合起来。595 nm 处反应产物的颜色强度更改与样品中的草酸盐成正比。
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Natalia Stepanova et al.
In vivo (Athens, Greece), 36(2), 925-933 (2022-03-05)
Little evidence is available on oxalate balance in peritoneal dialysis (PD) patients. We performed a cross-sectional observational pilot study with 62 adult PD patients to document oxalate balance and explore its association with PD-related peritonitis. Plasma oxalate concentration, levels of
Natalia Stepanova et al.
The Korean journal of internal medicine (2021-06-26)
It was hypothesized that oxalate might be strongly involved in atherogenesis and the inflammatory pathway that could result in an increased risk of cardiovascular disease (CVD) in end-stage renal disease (ESRD) patients. Therefore, this study aimed to address two primary
Natalia Stepanova et al.
Urolithiasis, 50(3), 249-258 (2022-02-08)
The present study aimed (i) to evaluate whether ceftriaxone treatment could affect not only intestinal oxalate-degrading bacteria number but also their total activity to degrade oxalate and influence oxalate homeostasis in rats, (ii) and to estimate the ability of commercially
Anna Kletzmayr et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(8), 1903337-1903337 (2020-04-25)
Calcium oxalate (CaOx) crystal-induced nephropathies comprise a range of kidney disorders, for which there are no efficient pharmacological treatments. Although CaOx crystallization inhibitors have been suggested as a therapeutic modality already decades ago, limited progress has been made in the
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What is the purpose of acidifying the samples for longer-term storage?
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What is the approximate expiration time for these kits?
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Expiration dating is batch specific and should be determined from the lot specific Certificate of Analysis. Please review your batch specific Certificate of Analysis by searching your lot number in the DOCUMENTATION section of the Product Detail Page:
https://www.sigmaaldrich.com/product/sigma/MAK315#product-documentationHelpful?
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