This information is proprietary and cannot be disclosed.
推荐产品
用途
sufficient for 100 colorimetric or fluorometric tests
檢測方法
colorimetric
fluorometric
儲存溫度
−20°C
一般說明
糖原是一种葡萄糖支链聚合物，是动物体内主要短期能量储存分子。 糖原主要在肝脏和肌肉组织中合成，占肝脏重量的10%和肌肉重量的1-2%。 当肌肉糖原通常就被肌肉使用，而肝脏糖原是调节血液葡萄糖水平的重要缓冲液。 糖尿病和因为先天代谢缺陷导致的糖原储存疾病，会导致糖原代谢失调。
適合性
适合于确定各种组织，比如肝脏等和细胞培养（粘附或悬浮细胞）中糖原浓度。
原則
糖原代谢能够被偶联酶分析检测，生成的比色（570nm）/荧光 (λex = 535/λem = 587 nm)产物和葡萄含糖原量成正比。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Insulin Signaling in Bupivacaine-induced Cardiac ToxicitySensitization during Recovery and Potentiation by Lipid Emulsion
Fettiplace M R, et al.
Anesthesiology, 124(2), 428-\442-428-\442 (2016)
Madhuri S Salker et al.
Scientific reports, 7(1), 12612-12612 (2017-10-05)
Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that
Nady Golestaneh et al.
Journal of translational medicine, 14(1), 344-344 (2016-12-22)
Study of age related macular degeneration (AMD) has been hampered by lack of human models that represent the complexity of the disease. Here we have developed a human in vitro disease model of AMD to investigate the underlying AMD disease
Cassius E O Coombs et al.
Meat science, 134, 86-97 (2017-08-05)
This study evaluated the effect of chilled followed by frozen storage on lamb quality and safety parameters. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24 h post-mortem, and
Ni Zeng et al.
PloS one, 15(4), e0230044-e0230044 (2020-04-03)
LEFTY2 (endometrial bleeding associated factor; EBAF or LEFTYA), a cytokine released shortly before menstrual bleeding, is a negative regulator of cell proliferation and tumour growth. LEFTY2 down-regulates Na+/H+ exchanger activity with subsequent inhibition of glycolytic flux and lactate production in
-
What is the polymer size of glycogen used in the standard of the MAK016-1KT Glycogen Assay Kit?
1 answer-
Helpful?
-
-
Is it permissible to proceed with boiling the liver samples homogenized in NP40 Substitute assay regent, even though the protocol states that "Tissue (10 mg) can be homogenized in 100 uL of water on ice. Boil homogenates for 5 minutes to inactivate enzymes"?
1 answer-
It is not certain whether the samples prepared in the NP40 Substitute assay reagent will be compatible with this kit. However, one can proceed to the next step by boiling the homogenates for 10 minutes to inactivate enzymes. Following that, the mixture should be centrifuged at 18000 rpm for 10 minutes to remove insoluble material. Collect the supernatant for the assay.
Helpful?
-
-
What could be the reason for the varying cloudiness levels in dilutions of INS-1 beta cell lysate when using the glycogen assay kit (cat #MAK016)? The solution became cloudy after dilution, with different dilutions performed: 1:2, 1:5, and 1:10. The 1:2 dilution appeared cloudier than the 1:5 and 1:10 dilutions, and the 1:5 dilution was cloudier than the 1:10 dilution. The cell lysate was clear after centrifugation before adding it to the hydrolysis buffer.
1 answer-
The observed precipitation may be due to the detergent in the assay buffer when added to the sample. To prevent this, it is recommended to bring the assay buffer to room temperature before adding it. Dilution has shown to reduce turbidity, so using the dilution with the least turbidity is advisable. Testing the performance of this dilution with the standard curve would be beneficial. Considering the high acidity of the hydrolysis buffer, it is uncertain if the pH changes upon adding the lysate, potentially causing precipitation. Testing the pH of the lysate using a pH strip and adjusting it to around 4.5 with HCl, if necessary, while keeping in mind the confirmed pH of the cell lysate is around 7, can be done.
Helpful?
-
-
Is it possible to know information about compatibility with extreme pH, rather than with salt buffers etc...?
1 answer-
The kit is tested with cell culture supernatant, biological fluids, tissue, and urine. It has not been tested with a sample that has an extreme pH. It is not known how that would affect the assay performance, as it has not been tested. However, the assay does work for urine which can have a wide pH range from pH 5-8.
In the protocol, glycogen is extracted using first KOH and then further extracted using HCl. The precipitate is then dissolved in Development Buffer/Hydrolysis Buffer for analysis which is buffered for the assay.
If the pH is already very basic this may be fine as it could be adjusted to the correct pH but if it is very acidic then the extraction may be difficult since the first step is to raise the pH. The buffers in the kit are also pH dependent as well so downstream steps require a specific pH.
Helpful?
-
-
We are looking for a Colorimetric/Fluorometric assay to determine glycogen content in various mouse tissues samples. We came across this kit and would like to know how much sample is required for this assay (volume/well).
1 answer-
The minimum tissue requirement is 10mg in 200 uL. After homogenization and cleanup, a range of 2-50 uL of the sample will be added to each well. For unknown samples it is advised to test several sample dilutions to ensure readings are within linear range. Please see the link below for the Product Information Sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/157/372/mak016bul.pdfHelpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
