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Merck

MAK013

Sigma-Aldrich

葡萄糖和蔗糖检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

别名:

葡萄糖和蔗糖比色/荧光分析试剂盒

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
food and beverages

检测方法

colorimetric
fluorometric

相关疾病

endocrinological disorders, diabetes

储存温度

−20°C

一般描述

蔗糖是一种二糖,蔗糖酶水解葡萄糖和果糖。[1]葡萄糖是细胞呼吸中用于能量产生的主要碳水化合物。[2]当细胞摄取时,葡萄糖能够被糖酵解分解或聚合用于能量储存。[2]

应用

葡萄糖和蔗糖检测试剂盒已被用于:
  • 测定蔗糖、[3]有氧糖酵解和丙酮酸激酶活性[4]
  • 测定草莓中的蛋白质和糖 [5]
  • 测定小鼠血浆胰岛素和葡萄糖水平[6]
  • 分析血浆葡萄糖和血浆乳酸[7]并定量测定葡萄糖/蔗糖[8]

适用性

适合于各种生物样本,包括血清,血浆,其他体液,食物和生长因子。

原理

在该检测试剂盒中,葡萄糖被葡萄糖氧化酶氧化,生成的比色(570nm)/荧光 (λex = 535 nm/λem = 587 nm) 产物和葡萄糖含量成正比。为了测量蔗糖,会在反应中加入蔗糖酶,将蔗糖转化成葡萄糖和果糖。总葡萄糖减去自由葡萄糖就是蔗糖的浓度。该试剂盒检测线性范围,葡萄糖荧光分析是0.2-1.0nmole,葡萄糖比色分析是2-10nmole。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Aquatic Chronic 3 - Resp. Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Swee-Suak Ko et al.
International journal of molecular sciences, 21(17) (2020-08-30)
The moth orchid is an important ornamental crop. It is very sensitive to high light irradiation due to photoinhibition. In this study, young orchid tissue culture seedlings and 2.5" potted plants pretreated under blue light (BL, λmax = 450 nm)
Tinghui Li et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 158, 401-409 (2020-10-31)
Bydureon® (Bdn) is a once-weekly injectable long-acting release (LAR) product for adults with type 2 diabetes based on PLGA microspheres encapsulating the glucagon like peptide (GLP-1) analog, exenatide. Despite its widespread use in type 2 diabetes treatment, little information has
Fatemeh Kazeminasab et al.
Nutrition & metabolism, 18(1), 75-75 (2021-07-22)
Insulin resistance is associated with prediabetes and further progression to type 2 diabetes mellitus (T2DM). This study aims to investigate novel hepatic lncRNAs associated with key genes in insulin resistance in prediabetes. In the bioinformatics phase, we have collected screened
Production of Probiotic Biomass (Lactobacillus rhamnosus IS9) against Salmonella sp for Use as a Feed Supplement in Poultry
Bertrand T F, et al.
International Journal of Current Microbiology and Applied Sciences, 6(6), 3286-3298 (2017)
Rates of Spontaneous Cleavage of Glucose, Fructose, Sucrose, and Trehalose in Water, and the Catalytic Proficiencies of Invertase and Trehalas
Richard W and Yang Y
Journal of the American Chemical Society, 130(24), 7548-7549 (2008)

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