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M1882

Sigma-Aldrich

肌红蛋白 来源于马心脏

Myoglobin from equine heart
1 of 1 reviewers received a sample product or took part in a promotion

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

别名:

肌红蛋白 来源于马心脏

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250 MG
$229.00
1 G
$541.00
5 G
$2,160.00
10 G
$3,750.00

$229.00


预计发货时间2025年4月14日详情


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250 MG
$229.00
1 G
$541.00
5 G
$2,160.00
10 G
$3,750.00

About This Item

EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.61

$229.00


预计发货时间2025年4月14日详情


获取大包装报价

生物来源

equine heart

方案

≥90% (SDS-PAGE)

表单

essentially salt-free, lyophilized powder

铁含量

≥0.20%

技术

MALDI-MS: suitable

UniProt登记号

储存温度

−20°C

基因信息

horse ... MB(100054434)

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应用

来自马心脏的肌红蛋白适用于:
  • Beckman DU-50或Gilford 2400分光光度计的光谱测定[1]
  • 通过使用单通道衰减全反射傅里叶变换红外(ATR-FT-IR)显微镜对
  • H2O溶液中蛋白质的二级结构进行分析[2]
  • 电喷雾质谱质量标度的校准(浓度为2 pmol/μL)[3]
  • 研究用于MALDI质谱的在线单液滴沉积[4]
  • 检测融合二氧化硅和聚丙烯酰胺涂层毛细血管中蛋白质的吸附[5]

生化/生理作用

肌红蛋白是在红色肌肉和心脏细胞中产生的氧的移动载体。该过程是对运动过程中氧气需求增加的反应,并可将氧气从肌膜传递至脊椎动物心脏和红色肌肉细胞的线粒体中。[6]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Guadalupe Gómez-Baena et al.
Scientific reports, 9(1), 10757-10757 (2019-07-26)
Major urinary proteins (MUP) are the major component of the urinary protein fraction in house mice (Mus spp.) and rats (Rattus spp.). The structure, polymorphism and functions of these lipocalins have been well described in the western European house mouse
D H Robertson et al.
Rapid communications in mass spectrometry : RCM, 11(7), 786-790 (1997-01-01)
Major urinary proteins (MUPs) from the urine of individual wild mice were characterized using electrospray ionization mass spectrometry (ESI-MS) and compared to MUPs from the urine of inbred mice. The wild mice showed considerable variation between individuals in the expression
Computer-controlled perifusion system for neuroendocrine tissues: development and applications.
A Negro-Vilar et al.
Methods in enzymology, 124, 67-79 (1986-01-01)
V Kery et al.
The Journal of biological chemistry, 269(41), 25283-25288 (1994-10-14)
The first committed step of transsulfuration is catalyzed by cystathionine beta-synthase (CBS), a known pyridoxal 5'-phosphate (PLP) enzyme. The inferred amino acid sequences of rat liver CBS and rat liver hemoprotein H-450 are identical. We now confirm the presence of
Brandye M Smith et al.
Analytical chemistry, 74(16), 4076-4080 (2002-08-30)
The application of single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy was investigated for secondary structure analysis of 15 representative proteins in H2O solution. This is the first reported application of single-pass ATR-FT-IR for protein analysis; thus, the method

商品

Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.

Chromatograms

application for HPLC

Questions

1–7 of 7 Questions  
  1. Is equine myoglobin (M1882) detectable and quantifiable by common laboratory tests (ECL) for the detection of human myoglobin?

    1 answer
    1. This item has not been tested in-house for detectability and quantifiability by common laboratory tests. The specifications for this item do not include testing by these methods.

      Helpful?

  2. Do you have the sequence for Product M1882, Myoglobin from equine heart?

    1 answer
    1. Product M1882 - Myoglobin from equine heart is purified from equine heart.  It is not sequenced.Accession P68082 for equine myoglobin has the following sequenceMGLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE DLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQGI hope that you find this information helpful.If you have further questions, please reply to this email.Sincerely,Audrey FlemingSigma-Aldrich Technical Service

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Is Product M1882, Myoglobin from equine heart, oxidized?

    1 answer
    1. Yes, it is oxidized.

      Helpful?

  5. Is Product M1882, Myoglobin from equine heart, metmyoglobin?

    1 answer
    1. The M1882 is a mixture, but we have not analyzed the percentages of the oxidized myoglobin or the reduced myoglobin in the product.

      Helpful?

  6. Which has more affinity for oxygen, hemoglobin or myoglobin?

    1 answer
    1. The affinity of myoglobin for oxygen is higher than that of hemoglobin.

      Helpful?

  7. What is the molecular weight of Product M1882, Myoglobin from equine heart?

    1 answer
    1. Based on the amino acid sequence (16,950) plus the heme group (616), the molecular weight is approximately 17,600 g/mol.

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Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. New York
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Is the myoglobin from equine heart dispersive in water?
    Or what are the solvent where it is dispersive.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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