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一般描述
乳酸氧化酶（LOX）是一种黄素单核苷酸（FMN）依赖型酶，是α-羟酸氧化酶黄素酶（flavoenzyme）家族一员。
应用
来自绿色气球菌 的乳酸氧化酶已被用于：
- 作为培养基增补剂用于将胞外乳酸转化为丙酮酸
- 涂布在玻璃珠表面用于以推拉式喷头系统（push-pull nozzle system）进行单个细胞的乳酸电化学检测
- 涂布在多枝化金纳米颗粒（GNP）表面用于以纸基多路复用设备（multiplexed device）同时比色测定唾液中的乳酸
生化/生理作用
在FMN辅助因子存在的情况下，乳酸氧化酶（LOX）可催化乳酸氧化形成丙酮酸和过氧化氢。该酶通过测定反应中形成的过氧化氢，有助于间接测定L-乳酸。
单位定义
一个单位定义为在标准测定条件下，在 37℃ 下，每分钟产生 1 mmol 过氧化氢的酶量。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Ingar Leiros et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 12), 1185-1190 (2006-12-05)
The crystal structure of L-lactate oxidase (LOX) from Aerococcus viridans has been determined at 2.1 A resolution. LOX catalyzes the flavin mononucleotide (FMN) dependent oxidation of lactate to pyruvate and hydrogen peroxide. LOX belongs to the alpha-hydroxy-acid oxidase flavoenzyme family;
Yashwanth Ashok et al.
PloS one, 15(2), e0223870-e0223870 (2020-02-25)
Lactate oxidases belong to a group of FMN-dependent enzymes and they catalyze a conversion of lactate to pyruvate with a release of hydrogen peroxide. Hydrogen peroxide is also utilized as a read out in biosensors to quantitate lactate levels in
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