Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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等级
Molecular Biology
for molecular biology
无菌性
sterile; autoclaved
表单
plates (10 cm)
plates (10cm, 20-25ml each)
存货情况
available only in USA
容量
20-25 mL
储存温度
2-8°C
适用性
selective for Escherichia coli
selective for coliforms
一般描述
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
应用
LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.[1]
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.
特点和优势
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
组分
15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin
其他说明
The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.
相关产品
产品编号
说明
价格
储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
其他客户在看
Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
Iacoviello Michael P and Rubin SA
Biotechniques, 30(5), 963-965 (2001)
商品
基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
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