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形狀
liquid
用途
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
特點
dNTPs included
hotstart
儲存條件
protect from light
技術
qPCR: suitable
顏色
colorless
輸入
purified DNA
相容性
for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5
檢測方法
SYBR® Green
運輸包裝
dry ice
儲存溫度
−20°C
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一般說明
高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。
應用
- 基因表达
- DNA定量
- 染色质免疫沉淀(CHiP)
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
特點和優勢
- 只需33分钟即可获得分析结果
- 高效、灵敏的实时PCR结果
- 几乎不需要多少优化，甚至无需优化
成分
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
其他說明
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
法律資訊
推薦
相關產品
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
商品
传统的PCR完成后，会通过琼脂糖凝胶——最近更常使用的是毛细管电泳系统——来分辨并分析数据。
PCR测定指南可指导您全程了解引物验证和其他测定优化因素，确保实现理想的DNA / RNA定量高灵敏度和特异性。
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
实验方案
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
相关内容
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
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