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Merck

K4002

Sigma-Aldrich

Krebs-Ringer碳酸氢盐缓冲液

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

别名:

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

分類程式碼代碼:
12161702
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

pH值

6.1-6.7

溶解度

water: 9.5 g/L, clear

儲存溫度

2-8°C

一般說明

Krebs-Ringer碳酸氢盐缓冲液作为盐溶液在组织培养中起重要作用。盐溶液用于供给细胞水和无机离子。还可维持培养基的渗透平衡和理想的pH水平。这种碳酸氢盐缓冲等渗盐水含有镁、钾、钠和磷酸根离子。

應用

Krebs-Ringer碳酸氢盐缓冲液已用于:

  • 作为洗涤缓冲液,用于原代脂肪细胞的制备和脂解分析
  • 作为预灌流溶液组分,用于胰岛活力和功能研究
  • 垂体的生物学分析

數量

配方中每升培养基含有9.5克的粉末。

重構

并补充1.26g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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