K0879

硫酸卡那霉素（来自卡那霉素链霉菌）

powder, γ-irradiated

• 别名: 卡那霉素 硫酸酯 来源于卡那霉素链霉菌, 卡那霉素 A, 卡那霉素碱 硫酸盐
• 经验公式（希尔记法）: C₁₈H₃₆N₄O₁₁ · H₂O₄S
• CAS号: 25389-94-0
• 分子量: 582.58
• EC號碼: 246-933-9
• MDL號碼: MFCD00070253
• 分類程式碼代碼: 12352200
• PubChem物質ID: 57654313
• NACRES: NA.85

属性

• 生物源: Streptomyces kanamyceticus
• 等級: for molecular biology
• 無菌: γ-irradiated
• 形狀: powder
• 效力: ≥700 μg per mg
• 顏色: white to off-white
• pH值: 6.5-8.5(1% solution)
• 抗生素活性譜: Gram-negative bacteria; Gram-positive bacteria; mycoplasma
• 作用方式: protein synthesis | interferes
• 儲存溫度: 2-8°C
• 適合性: nonselective for Escherichia coli; nonselective for coliforms
• SMILES 字串: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• InChI 密鑰: OOYGSFOGFJDDHP-KMCOLRRFSA-N

说明

一般說明

Chemical structure: aminoglycoside

Kanamycin, A from Streptomyces kanamyceticus is a polycationic aminoglycoside and a broad-spectrum antibiotic. Structurally, it comprises hydroxyl and amino functional groups.

應用

Kanamycin sulfate Streptomyces kanamyceticus has been used as an antibiotic component in Murashige and Skoog medium for the selection of tobacco tissue culture explants. It has also been used as an antibiotic in Luria broth for culturing Escherichia coli K-12 strain MG1655 cells for secretion assay and E. coli TV1061 cells for immobilization studies.

生化／生理作用

Kanamycin′s hydroxyl and amino functional groups are crucial for its membrane action functionality. However, it also exhibits toxicity towards mammalian cells. It prevents contamination in cell culture as well as a selective agent for bacteria with kanamycin resistance.

作用机制：该产品通过与70S核糖体亚基结合，抑制易位并引起错误编码而起作用。

耐药机制：氨基糖苷类修饰酶（包括乙酰转移酶、磷酸转移酶、核苷酸转移酶）可以改变这种抗生素，阻止其与核糖体相互作用。

抗菌谱：硫酸卡那霉素对革兰氏阴性和革兰氏阳性菌以及支原体有效。

原則

Kanamycin binds to the 30S ribosomal subunit and inhibits protein synthesis, leading to cell death.

準備報告

直接在小瓶中用无菌水配制储备液 (10mg/mL)。储备液应储存在 2-8°C 下。在 37°C 下可稳定 5 天。

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H360D
• 防範說明: P201 - P308 + P313
• 危險分類: Repr. 1B
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 2
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)